Mechanically-primed voltage-gated proton channels from angiosperm plants.
Mechanically-primed voltage-gated proton channels from angiosperm plants.
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DOI：
10.1038/s41467-023-43280-5
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发表时间：
2023-11-18
影响因子：
16.6
通讯作者：
Tombola, Francesco
中科院分区：
文献类型：
--
作者：
Zhao, Chang;Webster, Parker D.;De Angeli, Alexis;Tombola, Francesco
电压门控离子通道和机械门控离子通道是不同类别的膜蛋白，它们分别通过电刺激或机械刺激传导离子穿过门控孔并打开。在这里，我们描述了一个Hv通道（又名电压依赖性H+通道）从被子植物A。Thaliana具有独特的通道，因为只有在暴露于机械刺激后，它才会被电刺激打开，这个过程我们称之为启动。该通道定位于维管组织中，并且在维管植物中具有同源物。我们发现，机械启动是不需要激活的非被子植物HVS。在人工智能生成的植物Hv同系物结构模型的指导下，我们确定了一组在机械引发中起关键作用的残基。我们建议，HVS从被子植物需要启动，因为网络的亲水性/带电残基，锁定在一个沉默的静息构象的通道。机械刺激使网络不稳定，允许传导通路打开。与许多其他通道和受体相比，Hv蛋白被认为不具有失活或脱敏等机制。我们的研究结果表明，被子植物HV通道是电沉默，直到机械刺激打开其电压依赖性活动。作者描述了拟南芥脉管系统中的一种质子通道蛋白，它需要机械和电刺激才能开启。
Voltage-gated and mechanically-gated ion channels are distinct classes of membrane proteins that conduct ions across gated pores and are turned on by electrical or mechanical stimuli, respectively. Here, we describe an Hv channel (a.k.a voltage-dependent H+ channel) from the angiosperm plant A. thaliana that gates with a unique modality as it is turned on by an electrical stimulus only after exposure to a mechanical stimulus, a process that we call priming. The channel localizes in the vascular tissue and has homologs in vascular plants. We find that mechanical priming is not required for activation of non-angiosperm Hvs. Guided by AI-generated structural models of plant Hv homologs, we identify a set of residues playing a crucial role in mechanical priming. We propose that Hvs from angiosperm plants require priming because of a network of hydrophilic/charged residues that locks the channels in a silent resting conformation. Mechanical stimuli destabilize the network allowing the conduction pathway to turn on. In contrast to many other channels and receptors, Hv proteins are not thought to possess mechanisms such as inactivation or desensitization. Our findings demonstrate that angiosperm Hv channels are electrically silent until a mechanical stimulation turns on their voltage-dependent activity. The authors describe a proton channel protein in the vasculature of Arabidopsis thaliana that requires both mechanical and electrical stimuli to turn on. A mechanistic analysis identifies the molecular determinants for the hybrid activation process.
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影响因子：
33.6
作者：
DeCoursey TE
通讯作者：
DeCoursey TE
影响因子：
16.2
作者：
Aggarwal, SK;MacKinnon, R
通讯作者：
MacKinnon, R
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7.4
作者：
Guo, Qi;Liu, Lei;Rupasinghe, Thusitha W. T.;Roessner, Ute;Barkla, Bronwyn J.
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Barkla, Bronwyn J.
DOI：
10.1073/pnas.2119258119
发表时间：
2022-01-04
影响因子：
11.1
作者：
Bacete L;Schulz J;Engelsdorf T;Bartosova Z;Vaahtera L;Yan G;Gerhold JM;Tichá T;Øvstebø C;Gigli-Bisceglia N;Johannessen-Starheim S;Margueritat J;Kollist H;Dehoux T;McAdam SAM;Hamann T
通讯作者：
Hamann T
影响因子：
5.8
作者：
Wu, Xin;Zhang, Lu;Hong, Liang
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Hong, Liang