Correlation between Ureaplasma subgroup 2 and genitourinary tract disease outcomes revealed by an expanded multilocus sequence typing (eMLST) scheme.
Correlation between Ureaplasma subgroup 2 and genitourinary tract disease outcomes revealed by an expanded multilocus sequence typing (eMLST) scheme.
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DOI：
10.1371/journal.pone.0104347
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Xie X
中科院分区：
文献类型：
--
作者：
Zhang J;Kong Y;Ruan Z;Huang J;Song T;Song J;Jiang Y;Yu Y;Xie X
脲原体的多位点序列分型（MLST）方案基于4个管家基因（ftsH、rpL 22、valS和thrS），在我们以前的研究中描述了该方案，在此我们引入了一个具有改进的区分能力的扩展MLST（eMLST）方案，该方案通过在原始MLST方案中添加两个推定的毒力基因（ureG和mba-np 1）而开发。为了评价eMLST的区分能力，共研究了14株脲原体血清型参考菌株和269株临床菌株（134株分离自症状性患者，135株分离自无症状患者）。我们的研究证实，所有14种血清型菌株都可以成功区分为14种eMLST ST（eST），而其中一些甚至无法通过MLST区分，在我们调查的临床分离株中总共鉴定出136种eST。此外，系统发育分析表明，两个遗传上显着遥远的集群（集群I和II），大多数临床分离株位于集群I。这些发现与我们先前研究中两个著名的遗传谱系（微小脲原体和解脲脲原体）的概念一致，并进一步支持这一概念。有趣的是，尽管两个簇都与临床表现相关，但簇II的亚组2对患者有明显的不良影响，可能是临床结局的潜在风险因素。总之，eMLST方案为研究者提供了一种高度区分性的分型工具，能够进行精确的流行病学调查和支原体的临床相关性。
The multilocus sequence typing (MLST) scheme of Ureaplasma based on four housekeeping genes (ftsH, rpL22, valS, and thrS) was described in our previous study; here we introduced an expanded MLST (eMLST) scheme with improved discriminatory power, which was developed by adding two putative virulence genes (ureG and mba-np1) to the original MLST scheme. To evaluate the discriminatory power of eMLST, a total of 14 reference strains of Ureaplasma serovars and 269 clinical strains (134 isolated from symptomatic patients and 135 obtained from asymptomatic persons) were investigated. Our study confirmed that all 14 serotype strains could successfully be differentiated into 14 eMLST STs (eSTs), while some of them could not even be differentiated by the MLST, and a total of 136 eSTs were identified among the clinical isolates we investigated. In addition, phylogenetic analysis indicated that two genetically significantly distant clusters (cluster I and II) were revealed and most clinical isolates were located in cluster I. These findings were in accordance with and further support for the concept of two well-known genetic lineages (Ureaplasma parvum and Ureaplasma urealyticum) in our previous study. Interestingly, although both clusters were associated with clinical manifestation, the sub-group 2 of cluster II had pronounced and adverse effect on patients and might be a potential risk factor for clinical outcomes. In conclusion, the eMLST scheme offers investigators a highly discriminative typing tool that is capable for precise epidemiological investigations and clinical relevance of Ureaplasma.
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影响因子：
5.3
作者：
Shariat, Nikki;DiMarzio, Michael J.;Dudley, Edward. G.
通讯作者：
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