Roles of Two Sox9 Genes during Gonadal Development in Japanese Flounder: Sex Differentiation, Spermatogenesis and Gonadal Function Maintenance.
Roles of Two Sox9 Genes during Gonadal Development in Japanese Flounder: Sex Differentiation, Spermatogenesis and Gonadal Function Maintenance.
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两个 Sox9 基因在牙鲆性腺发育过程中的作用：性别分化、精子发生和性腺功能维持
DOI：
10.3390/ijms19020512
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发表时间：
2018-02-08
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Li X;Yu H;Wang Y;Liu X;Liu Y;Qu J;Wang X
转录因子sox9与哺乳动物的软骨形成和睾丸决定有关。在日本比目鱼（Paralichthys olivaceus）中发现了两个重复的sox9基因，分别命名为Posox9a和Posox9b。系统发育和基因结构分析表明，Posox9a和Posox9b与硬骨鱼和四足动物同源。定量实时聚合酶链反应（qRT-PCR）结果显示，Posox9a和Posox9b在睾丸组织中的表达均高于卵巢组织。性腺原位杂交（ISH）分析显示，Posox9a和Posox9b mRNA均存在于卵母细胞、支持细胞和精母细胞中。在性别分化过程中，Posox9a的表达从孵化后60天开始表现出明显的两性二态表达，雄偏好个体的表达量高于雌偏好个体，从30天到100天逐渐增加。在Posox9b表达中也检测到类似的模式。注射不同浓度雄激素（17α-甲基睾酮）后，Posox9b的表达水平显著升高，而Posox9a的表达水平变化不明显。上述结果表明，牙鲆两种sox9基因在性别分化中具有相反的功能，但在17α-甲基睾酮给药方面存在明显差异，值得进一步探讨其进化和适应意义。本研究为进一步探讨Posox9基因在牙鲆性别决定与分化、精子发生和性腺功能维持中的作用奠定了基础。
The transcription factor sox9 has been implicated in cartilage formation and testis determination in mammals. Here, two duplicates of sox9 were found in Japanese flounder (Paralichthys olivaceus) named Posox9a and Posox9b, respectively. Phylogenetic and gene structure analyses revealed that Posox9a and Posox9b were homologous to that of teleosts and tetrapods. Quantitative real-time polymerase chain reaction (qRT-PCR) showed that both Posox9a and Posox9b expressed higher in testis than in ovary of adult tissues. The in situ hybridization (ISH) analysis of gonads showed that Posox9a and Posox9b mRNA were both detected in oocytes, Sertoli cells and spermatocytes. During sex differentiation, the expression of Posox9a exhibited obvious sexual dimorphic expression from 60 days after hatch (dah) with higher expression in male preferred individuals than female preferred individuals and increased gradually from 30 to 100 dah. A similar pattern was detected in Posox9b expression. After injection of androgen (17α-methyltestosterone) of different concentrations, the expression level of Posox9b increased significantly, whereas Posox9a did not change obviously. These results indicated that the two sox9 genes of Japanese flounder had converse functions in sex differentiation, whereas their differences in 17α-methyltestosterone administration were obvious and worthwhile for exploring evolutionary and adaptive significance. This study provided a foundation for further exploration of the roles of Posox9 genes during the sex determination and differentiation, spermatogenesis and gonadal function maintenance of Japanese flounder.
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日本牙鲆 (Paralichthys olivaceus) 中 Nanog 同源物的鉴定和表征
影响因子：
3.5
作者：
Gao, Jinning;Wang, Jing;Wang, Xubo
通讯作者：
Wang, Xubo
DOI：
10.1534/g3.114.012138
发表时间：
2014-11-05
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Wang W;Wang J;You F;Ma L;Yang X;Gao J;He Y;Qi J;Yu H;Wang Z;Wang X;Wu Z;Zhang Q
通讯作者：
Zhang Q
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影响因子：
1.4
作者：
Dong Xiaoli;Chen Songlin;Shao Changwei
通讯作者：
Shao Changwei
影响因子：
2.7
作者：
Chiang, EFL;Pai, CI;Chung, BC
通讯作者：
Chung, BC
影响因子：
1.6
作者：
Shen Xueyan;Cui Jianzhou;Gu Qianqun
通讯作者：
Gu Qianqun