Stratified active archaeal communities in the sediments of Jiulong River estuary, China.
Stratified active archaeal communities in the sediments of Jiulong River estuary, China.
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中国九龙江口沉积物中分层的活跃古菌群落
DOI：
10.3389/fmicb.2012.00311
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发表时间：
2012
影响因子：
5.2
通讯作者：
Xiao X
中科院分区：
文献类型：
--
作者：
Li Q;Wang F;Chen Z;Yin X;Xiao X
本文报道了福建九龙江河口沉积物岩心中总古菌群落和活跃古菌群落的组成。沉积物岩心CH4和SO2−4浓度分布表明存在硫酸盐-甲烷过渡带（SMTZ），在该过渡带中发生硫酸盐还原耦合甲烷厌氧氧化（AOM）。据此，从1.2 m沉积物岩心处选取16 ~ 18.5 cm、71 ~ 73.5 cm和161 ~ 163.5 cm 3个沉积层，分别命名为顶、中、底。提取各层总DNA和RNA，用于16S rRNA基因、16S rRNA反转录(RT)-PCR产物和甲基CoM还原酶α亚基（mcrA）基因的克隆文库和序列分析。系统发育分析表明，三层古菌群落以杂绿古菌群（Miscellaneous Crenarchaeotal Group， MCG）为主，其生态功能尚不清楚。MCG- a、B、C、D、E、F、g为7个亚群。MCG- a和MCG- g是河口沉积物中最活跃的亚群。已知的厌氧甲烷营养古菌（ANMEs）在这些河口古菌群落中只占很小的组成部分。这项研究，连同对深地下沉积物的研究，将是一个很好的起点，以确定和比较特定的活跃古细菌群及其在黑暗、深地下沉积物环境中的作用。
Here the composition of total and active archaeal communities in a sediment core of Jiulong River estuary at Fujian Province, Southern China was reported. Profiles of CH4 and SO2−4 concentrations from the sediment core indicated the existence of a sulfate-methane transition zone (SMTZ) in which sulfate reduction-coupled anaerobic oxidation of methane (AOM) occurs. Accordingly, three sediment layers (16–18.5 cm, 71–73.5 cm, and 161–163.5 cm) from the 1.2 m sediment core were sectioned and named top, middle and bottom, respectively. Total DNA and RNA of each layer were extracted and used for clone libraries and sequence analysis of 16S rRNA genes, the reverse transcription (RT)-PCR products of 16S rRNA and methyl CoM reductase alpha subunit (mcrA) genes. Phylogenetic analysis indicated that archaeal communities of the three layers were dominated by the Miscellaneous Crenarchaeotal Group (MCG) whose ecological functions were still unknown. The MCG could be further divided into seven subgroups, named MCG-A, B, C, D, E, F, and G. MCG-A and MCG-G were the most active groups in the estuarine sediments. Known anaerobic methanotrophic archaea (ANMEs) were only found as minor components in these estuarine archaeal communities. This study, together with the studies of deep subsurface sediments, would be a very good start point to target and compare the specific active archaeal groups and their roles in the dark, deep subsurface sediment environments.
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影响因子：
14.9
作者：
Ludwig, W;Strunk, O;Schleifer, KH
通讯作者：
Schleifer, KH
DOI：
10.1073/pnas.0600035103
发表时间：
2006-03-07
影响因子：
11.1
作者：
Biddle, JF;Lipp, JS;Hinrichs, KU
通讯作者：
Hinrichs, KU
影响因子：
4.4
作者：
Knittel, K;Lösekann, T;Amann, R
通讯作者：
Amann, R
影响因子：
4.4
作者：
Dilly, O;Bloem, J;Munch, JC
通讯作者：
Munch, JC
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M