Lactoferrin/Calcium Phosphate-Modified Porous Ti by Biomimetic Mineralization: Effective Infection Prevention and Excellent Osteoinduction.
Lactoferrin/Calcium Phosphate-Modified Porous Ti by Biomimetic Mineralization: Effective Infection Prevention and Excellent Osteoinduction.
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乳铁蛋白/磷酸钙仿生矿化改性多孔钛：有效预防感染和出色的骨诱导作用
DOI：
10.3390/ma14040992
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发表时间：
2021-02-19
期刊：
影响因子：
--
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Chen S;He Y;Zhong L;Xie W;Xue Y;Wang J
钛表面改性可以提高种植体的骨结合能力和抗菌性能。在这项研究中，我们用磷酸钙（CaP）和不同浓度的乳铁蛋白（LF）仿生矿化修饰多孔钛盘，并检查其抗菌效果和成骨生物活性。首先，采用扫描电镜（SEM）、荧光示踪法、X射线光电子能谱（XPS）、傅里叶变换红外光谱（FTIR）、能量色散X射线能谱（EDX）、X射线衍射（XRD）和LF释放动力学等方法对改性后的Ti表面进行表征。然后，研究了对S. sanguis和S.金黄色葡萄球菌进行了研究。最后，进行体外细胞学检查，包括评价细胞粘附、细胞分化、细胞外基质矿化和细胞毒性。结果表明，CaP和LF均能成功修饰多孔钛片，LF-M组（200 μg/mL LF在模拟体液中）可控制LF的轻度释放。LF-M组能有效抑制S. sanguis和S.金黄色葡萄球菌，促进体外成骨分化，具有良好的生物相容性。因此，LF-M改性钛可能在牙科种植体领域具有潜在的应用，以促进骨整合并预防种植体周围炎的发生。
The surface modification of titanium (Ti) can enhance the osseointegration and antibacterial properties of implants. In this study, we modified porous Ti discs with calcium phosphate (CaP) and different concentrations of Lactoferrin (LF) by biomimetic mineralization and examined their antibacterial effects and osteogenic bioactivity. Firstly, scanning electron microscopy (SEM), the fluorescent tracing method, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and the releasing kinetics of LF were utilized to characterize the modified Ti surface. Then, the antibacterial properties against S. sanguis and S. aureus were investigated. Finally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell differentiation, extracellular matrix mineralization, and cytotoxicity. The results showed that the porous Ti discs were successfully modified with CaP and LF, and that the LF-M group (200 μg/mL LF in simulated body fluid) could mildly release LF under control. Further, the LF-M group could effectively inhibit the adhesion and proliferation of S. sanguis and S. aureus and enhance the osteogenic differentiation in vitro with a good biocompatibility. Consequently, LF-M-modified Ti may have potential applications in the field of dental implants to promote osseointegration and prevent the occurrence of peri-implantitis.
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影响因子：
1.1
作者：
Legostaeva, E. V.;Kulyashova, K. S.;Khlusov, I. A.
通讯作者：
Khlusov, I. A.
影响因子：
7.6
作者：
Lang, N. P.
通讯作者：
Lang, N. P.
影响因子：
4.3
作者：
Abrahamsson, I;Berglundh, T;Lindhe, J
通讯作者：
Lindhe, J
DOI：
10.1016/j.bbrc.2018.10.193
发表时间：
2018-12-09
影响因子：
3.1
作者：
Chea, Chanbora;Miyauchi, Mutsumi;Takata, Takashi
通讯作者：
Takata, Takashi
影响因子：
3.4
作者：
Balasubramanian, Sivaraman Arumugam;Pye, David Cecil;Willcox, Mark Duncan Perry
通讯作者：
Willcox, Mark Duncan Perry