The Power of Touch: Type 4 Pili, the von Willebrand A Domain, and Surface Sensing by Pseudomonas aeruginosa.
The Power of Touch: Type 4 Pili, the von Willebrand A Domain, and Surface Sensing by Pseudomonas aeruginosa.
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DOI：
10.1128/jb.00084-22
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发表时间：
2022-06-21
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
生物圈中的大多数微生物都附着在表面上，在那里它们由于流体动力流动和细胞与基质的相互作用而受到机械力。这些力可能作为影响细菌生理的机械线索，最终驱动环境适应和适应性。机械传感器是能够感知机械输入的细胞部件，并作为传感和传导机械信号的大系统的一部分。细菌中的两种细胞成分已成为候选的机械传感器是IV型毛（TFP）和鞭毛。目前的模型假设细菌将TFP和/或鞭毛依赖的机械力输入传递并转化为生化信号，包括cAMP和c-di-GMP，以驱动表面适应。在这里，我们讨论了力诱导的变化对两种真核蛋白（titin和人血管性血液病因子（vWF））的结构和功能的影响，以及这些蛋白与细菌的相关性。鉴于对这些真核生物机械传感器的丰富了解，我们可以将它们作为一个框架来理解在生物膜形成的早期阶段力对铜绿假单胞菌的影响，特别强调TFP和记录的表面传感机械传感器PilY1和FimH。我们还讨论了二硫键在介导力诱导构象变化中的重要性，这可能调节机械传感和下游生化信号。最后，我们分享了我们对该领域现状的看法，以及我们认为在研究细菌机械传感方面令人兴奋的前沿，以更好地理解细菌从浮游生物向生物膜生活方式转变的机制。
Most microbes in the biosphere are attached to surfaces, where they experience mechanical forces due to hydrodynamic flow and cell-to-substratum interactions. These forces likely serve as mechanical cues that influence bacterial physiology and eventually drive environmental adaptation and fitness. Mechanosensors are cellular components capable of sensing a mechanical input and serve as part of a larger system for sensing and transducing mechanical signals. Two cellular components in bacteria that have emerged as candidate mechanosensors are the type IV pili (TFP) and the flagellum. Current models posit that bacteria transmit and convert TFP- and/or flagellum-dependent mechanical force inputs into biochemical signals, including cAMP and c-di-GMP, to drive surface adaptation. Here, we discuss the impact of force-induced changes on the structure and function of two eukaryotic proteins, titin and the human von Willebrand factor (vWF), and these proteins’ relevance to bacteria. Given the wealth of understanding about these eukaryotic mechanosensors, we can use them as a framework to understand the effect of force on Pseudomonas aeruginosa during the early stages of biofilm formation, with a particular emphasis on TFP and the documented surface-sensing mechanosensors PilY1 and FimH. We also discuss the importance of disulfide bonds in mediating force-induced conformational changes, which may modulate mechanosensing and downstream biochemical signaling. We conclude by sharing our perspective on the state of the field and what we deem exciting frontiers in studying bacterial mechanosensing to better understand the mechanisms whereby bacteria transition from a planktonic to a biofilm lifestyle.
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DOI：
10.1126/science.aad2001
发表时间：
2016-03-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chang YW;Rettberg LA;Treuner-Lange A;Iwasa J;Søgaard-Andersen L;Jensen GJ
通讯作者：
Jensen GJ
影响因子：
3.4
作者：
Bechtel TJ;Weerapana E
通讯作者：
Weerapana E
影响因子：
3.7
作者：
Cheng G;Tse J;Jain RK;Munn LL
通讯作者：
Munn LL
影响因子：
3.8
作者：
Bonez, Pauline Cordenonsi;Rossi, Grazielle Guidolin;Anraku de Campos, Marli Matiko
通讯作者：
Anraku de Campos, Marli Matiko
影响因子：
9.2
作者：
Carniello, Vera;Peterson, Brandon W.;Busscher, Henk J.
通讯作者：
Busscher, Henk J.