Ambient ultrafine particles activate human monocytes: Effect of dose, differentiation state and age of donors.
Ambient ultrafine particles activate human monocytes: Effect of dose, differentiation state and age of donors.
复制标题
DOI：
10.1016/j.envres.2017.11.019
复制
发表时间：
2018-03
影响因子：
8.3
通讯作者：
Campbell A
中科院分区：
文献类型：
--
作者：
Bliss B;Tran KI;Sioutas C;Campbell A
Exposure to ambient particulate matter (PM) has been linked to adverse pulmonary and cardiovascular health effects. Activation of both inflammatory and oxidative stress pathways has been observed and may be a probable cause of these outcomes. We tested the hypothesis that in human monocytes, PM-induced oxidative and inflammatory responses are interrelated. A human monocytic cell line (THP-1) was used to determine if dose and differentiation state plays a role in the cellular response after a 24hr exposure to particles. Primary human monocytes derived from eight female, nonsmoker donors (aged: 21, 24, 27, 28, 48, 49, 54 & 60yo) were used to determine if the age of donors modulates the response. Cells were treated with aqueous suspensions of ambient ultrafine particles (UFP, defined as smaller than 0.2 μm in size) or a media control for 24hr. After exposure, reactive oxygen species (ROS) formation was increased irrespective of dose or differentiation state of THP-1 cells. In the primary human monocytes, ROS formation was not significantly changed. The release of the proinflammatory cytokine, tumor necrosis factor alpha (TNF-α), was dose-dependent and greatest in differentiated compared to undifferentiated THP-1 cells exposed to UFP. In the Primary human monocytes, TNF-α secretion was increased irrespective of the age of the donor. Our results suggest that after a 24hr exposure to particles, general reactive oxygen species formation was nonspecific and uncorrelated to cytokine secretion which was consistently enhanced. Cytokines play an important role in orchestrating many immune responses and thus the ability of ambient particles to enhance robust secretion of a proinflammatory cytokine from primary human monocytes, and how this may influence the response to pathogens and alter disease states, needs to be further evaluated.
登录
查看更多内容
DOI：
10.1056/nejmsa0805646
发表时间：
2009-01-22
期刊：
The New England journal of medicine
影响因子：
--
作者：
Pope CA 3rd;Ezzati M;Dockery DW
通讯作者：
Dockery DW
影响因子：
10
作者：
Araujo JA;Nel AE
通讯作者：
Nel AE
影响因子：
3.5
作者：
Bach, Nicolai;Bolling, Anette Kocbach;Ovrevik, Johan
通讯作者：
Ovrevik, Johan
影响因子：
5.8
作者：
Ishii H;Hayashi S;Hogg JC;Fujii T;Goto Y;Sakamoto N;Mukae H;Vincent R;van Eeden SF
通讯作者：
van Eeden SF
影响因子：
8.8
作者：
Bhavaraju, Laya;Shannahan, Jonathan;Madden, Michael
通讯作者：
Madden, Michael