Catalytic properties of RNA polymerases IV and V: accuracy, nucleotide incorporation and rNTP/dNTP discrimination.
Catalytic properties of RNA polymerases IV and V: accuracy, nucleotide incorporation and rNTP/dNTP discrimination.
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DOI：
10.1093/nar/gkx794
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发表时间：
2017-11-02
影响因子：
14.9
通讯作者：
Pikaard CS
中科院分区：
文献类型：
--
作者：
Marasco M;Li W;Lynch M;Pikaard CS
所有真核生物都有三种基本的核多亚单位RNA聚合酶，简称Pol I、Pol II和Pol III。植物特别是另外有两种多亚单位RNA聚合酶，Pol IV和Pol V，它们合成非编码RNA，协调RNA指导的DNA甲基化，以沉默转座子和一组基因。根据它们的亚基组成，POL IV和V显然演变为POL II的特殊形式，但它们的催化性能仍未确定。在这里，我们展示了POL IV和V以及POL II在核苷酸参入率、转录准确性以及区分核苷酸和脱氧核苷酸的能力方面的不同。Pol IV转录比Pol II或V更容易出错，后者在合成短RNA时可能是可以容忍的，这些短RNA是针对转座子家族不同成员的siRNA的前体。相比之下，Pol V表现出与Pol II相似的高保真转录，这表明Pol V转录本需要真实地反映siRNA-ArgAerte沉默复合体所在的靶基因座的DNA序列。
All eukaryotes have three essential nuclear multisubunit RNA polymerases, abbreviated as Pol I, Pol II and Pol III. Plants are remarkable in having two additional multisubunit RNA polymerases, Pol IV and Pol V, which synthesize noncoding RNAs that coordinate RNA-directed DNA methylation for silencing of transposons and a subset of genes. Based on their subunit compositions, Pols IV and V clearly evolved as specialized forms of Pol II, but their catalytic properties remain undefined. Here, we show that Pols IV and V differ from one another, and Pol II, in nucleotide incorporation rate, transcriptional accuracy and the ability to discriminate between ribonucleotides and deoxyribonucleotides. Pol IV transcription is considerably more error-prone than Pols II or V, which may be tolerable in its synthesis of short RNAs that serve as precursors for siRNAs targeting non-identical members of transposon families. By contrast, Pol V exhibits high fidelity transcription, similar to Pol II, suggesting a need for Pol V transcripts to faithfully reflect the DNA sequence of target loci to which siRNA–Argonaute silencing complexes are recruited.
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影响因子：
16
作者：
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