"Two-in-one" portable strategy for non-GOx glucose monitoring: Electrochromic and fluorescent assay using reversible hydrogel
"Two-in-one" portable strategy for non-GOx glucose monitoring: Electrochromic and fluorescent assay using reversible hydrogel
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用于非 Gox 葡萄糖监测的“二合一”便携式策略：使用可逆水凝胶进行电致变色和荧光测定
DOI：
10.1016/j.snb.2021.130744
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发表时间：
2021-12
影响因子：
8.4
通讯作者：
Liu Yingju
中科院分区：
文献类型：
--
作者：
Liang Hongzhi;Wang Jiancong;Qileng Aori;Chen Shanqin;Zhang Qianying;Liu Weipeng;Liu Yingju
葡萄糖的检测在临床诊断中具有重要意义，而传统的葡萄糖传感方法主要依赖于葡萄糖氧化酶（GOx）。本文利用聚乙烯硼酸酯（PVB）水凝胶制备简单、稳定性好、选择性好等优点，将其用于非葡萄糖氧化酶的便携式葡萄糖检测。在比色通道中，水凝胶在葡萄糖存在下可以特异性地分解，从而可以改变电阻，在3s的极短时间内引起电致变色普鲁士蓝（PB）膜的显著颜色变化。PB的灰度值与葡萄糖浓度在0.1 - 10.0 mM范围内呈线性关系，检测限（LOD）为0.02 mM。此外，PVB凝胶中的碱性磷酸酶（ALP）被释放出来，与L-抗坏血酸-2-磷酸反应，生成的抗坏血酸可还原HAuCl 4，形成Au纳米团簇的聚集诱导发射增强（AIEE）。实现了葡萄糖浓度为0.1-1.0 mM和1.0-10.0 mM的两个单独范围，以及0.018 mM的LOD。特别是设计了一个移动的APP来读取PB的灰度值，使传感器适合现场检测。总体而言，这项工作在低成本，稳定和便携式葡萄糖检测方面显示出巨大的潜力。
The detection of glucose is important in clinic diagnosis, while the traditional methods for glucose sensing rely heavily on glucose oxidase (GOx). Herein, with the easy preparation, stability as well as selectivity, a polyvinyl borate (PVB) hydrogel was used for the non-GOx and portable detection of glucose. In the colorimetric channel, the hydrogel can be specifically decomposed in the presence of glucose, thus the electric resistance can be changed, causing significant color change of the electrochromic Prussian blue (PB) film in a very short time of 3 s. A linear relationship between the grayscale value of PB and glucose concentration was established in the range between 0.1 and 10.0 mM with the limit of detection (LOD) of 0.02 mM. In addition, the alkaline phosphatase (ALP) in the PVB gel was released to react withL-ascorbyl-2-phosphate, thus the product of ascorbic acid can reduce HAuCl4to form the aggregation-induced emission enhancement (AIEE) of Au nanoclusters. Two separate ranges with glucose concentrations of 0.1–1.0 mM and 1.0–10.0 mM, and also a LOD of 0.018 mM were achieved. In particular, a mobile APP was designed to read the grayscale values of PB, making the sensor suitable for the on-site detection. Overall, this work may show a great potential in low-cost, stable and portable glucose detection.
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DOI：
10.1007/s11426-016-0279-3
发表时间：
2016
期刊：
Science China Chemistry
影响因子：
--
作者：
D. Zhou;D. Xie;X. Xia;Xiuli Wang;C. Gu;J. Tu
通讯作者：
D. Zhou;D. Xie;X. Xia;Xiuli Wang;C. Gu;J. Tu
影响因子：
4.7
作者：
Chaudhary, Anjali;Pathak, Devesh K.;Kumar, Rajesh
通讯作者：
Kumar, Rajesh
影响因子：
6.9
作者：
Alesanco, Yolanda;Vinuales, Ana;Tena-Zaera, Ramon
通讯作者：
Tena-Zaera, Ramon
水热合成氮硼共掺杂碳量子点，用于丙酮和多巴胺传感器和多色细胞成像
影响因子：
8.4
作者：
Liu, Yushan;Li, Wei;Liu, Shouxin
通讯作者：
Liu, Shouxin
DOI：
10.1073/pnas.1822007116
发表时间：
2019-06-25
影响因子：
11.1
作者：
Evans, R. Colby;Ellingworth, Austin;Sambur, Justin B.
通讯作者：
Sambur, Justin B.