Virulence factors of Moraxella catarrhalis outer membrane vesicles are major targets for cross-reactive antibodies and have adapted during evolution.
Virulence factors of Moraxella catarrhalis outer membrane vesicles are major targets for cross-reactive antibodies and have adapted during evolution.
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DOI：
10.1038/s41598-018-23029-7
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发表时间：
2018-03-21
影响因子：
4.6
通讯作者：
Mackiewicz P
中科院分区：
文献类型：
--
作者：
Augustyniak D;Seredyński R;McClean S;Roszkowiak J;Roszniowski B;Smith DL;Drulis-Kawa Z;Mackiewicz P
卡他莫拉菌是一种常见的人类呼吸道病原菌。其与全菌或外膜囊泡（OMV）相关的毒力因子有助于感染、定植，并可能诱导特异性抗体。为了研究病原体-宿主相互作用，我们应用了集成的生物信息学和免疫蛋白质组学（2D电泳，免疫印迹，LC-MS/MS）方法。我们发现，OMV蛋白只参与补体逃避和定殖策略，但不参与铁转运和代谢，是针对遗传上不同的M产生的交叉反应抗体的主要靶点。卡他菌株。对31个M.卡他莫拉菌和其它莫拉菌的研究表明，OMV蛋白编码基因分属于64个直链组，其中5个基因仅限于莫拉菌。粘膜炎。相对于其祖先和动物致病性莫拉氏菌，该物种的OMV蛋白编码基因数量增加了两倍。OMV特异性因子的出现和OMV毒力相关蛋白的增加是在M.卡他病的进化是病原体适应以优化定殖的有趣例子。这精确地靶向针对M的交叉反应性免疫。卡他病可能是宿主抵抗这种现象的重要策略。我们证明，交叉反应性是密切相关的抗毒力抗体库，我们已经联系到适应这种病原体的主机。
Moraxella catarrhalis is a common human respiratory tract pathogen. Its virulence factors associated with whole bacteria or outer membrane vesicles (OMVs) aid infection, colonization and may induce specific antibodies. To investigate pathogen-host interactions, we applied integrated bioinformatic and immunoproteomic (2D-electrophoresis, immunoblotting, LC-MS/MS) approaches. We showed that OMV proteins engaged exclusively in complement evasion and colonization strategies, but not those involved in iron transport and metabolism, are major targets for cross-reacting antibodies produced against phylogenetically divergent M. catarrhalis strains. The analysis of 31 complete genomes of M. catarrhalis and other Moraxella revealed that OMV protein-coding genes belong to 64 orthologous groups, five of which are restricted to M. catarrhalis. This species showed a two-fold increase in the number of OMV protein-coding genes relative to its ancestors and animal-pathogenic Moraxella. The appearance of specific OMV factors and the increase in OMV-associated virulence proteins during M. catarrhalis evolution is an interesting example of pathogen adaptation to optimize colonization. This precisely targeted cross-reactive immunity against M. catarrhalis may be an important strategy of host defences to counteract this phenomenon. We demonstrate that cross-reactivity is closely associated with the anti-virulent antibody repertoire which we have linked with adaptation of this pathogen to the host.
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