Clear cell papillary renal cell carcinoma: micro-RNA expression profiling and comparison with clear cell renal cell carcinoma and papillary renal cell carcinoma.
Clear cell papillary renal cell carcinoma: micro-RNA expression profiling and comparison with clear cell renal cell carcinoma and papillary renal cell carcinoma.
复制标题
DOI：
10.1016/j.humpath.2014.01.013
复制
发表时间：
2014-06
期刊：
影响因子：
3.3
通讯作者：
Netto, George J.
中科院分区：
文献类型：
--
作者：
Munari, Enrico;Marchionni, Luigi;Chitre, Apurva;Hayashi, Masamichi;Martignoni, Guido;Brunelli, Matteo;Gobbo, Stefano;Argani, Pedram;Allaf, Mohamad;Hoque, Mohammad O.;Netto, George J.
透明细胞乳头状肾细胞癌（CCPRCC）是一种低级别的肾脏肿瘤，其形态学特征类似于透明细胞肾细胞癌（CCRCC）和乳头状肾细胞癌（PRCC）。然而，尽管有一些重叠的功能，他们的形态，免疫组织化学和分子概况是不同的。micro-RNAs（miRNAs）是一种小的非编码RNA，在调节基因表达中起着至关重要的作用，并参与各种生物学过程，包括癌症的发展。为了更好地了解这种肿瘤的生物学特性，我们的目的是使用微阵列和定量逆转录聚合酶链反应分析一组CCPRCC的miRNA表达谱。共15例诊断为CCPRCC患者在这项研究中使用。在CCPRCC中差异表达最多的miRNA中，我们发现与正常肾实质相比，miR-210、miR-122、miR-34 a、miR-21、miR-34 b * 和miR-489上调，而miR-4284、miR-1202、miR-135 a、miR-1973和miR-204下调。为了鉴定CCPRCC、CCRCC和PRCC之间差异调节的miRNA的一致性，我们另外使用来自NCBI基因表达综合数据库（GSE 41282和GSE 3798）的2个可生物学获得的微阵列数据集确定差异miRNA表达。比较结果显示，CCPRCC与PRCC的miRNA表达谱存在一定的重叠。此外，CCPRCC缺乏通常与攻击行为相关的重要miRNA的失调。总之，我们描述了一种相对罕见的肾癌的miRNA表达谱。我们的研究结果可能有助于理解这种新认识的实体的分子基础。
Clear cell papillary renal cell carcinoma (CCPRCC) is a low-grade renal neoplasm with morphological characteristics mimicking both clear cell renal cell carcinoma (CCRCC) and papillary renal cell carcinoma (PRCC). However, despite some overlapping features, their morphological, immunohistochemical, and molecular profiles are distinct. Micro-RNAs (miRNAs) are small noncoding RNAs that play a crucial role in regulating gene expression and are involved in various biological processes, including cancer development. To better understand the biology of this tumor, we aimed to analyze the miRNA expression profile of a set of CCPRCC using microarray and quantitative reverse transcription–polymerase chain reaction. A total of 15 cases diagnosed as CCPRCC were used in this study. Among the most differentially expressed miRNA in CCPRCC, we found miR-210, miR-122, miR-34a, miR-21, miR-34b*, and miR-489 to be up-regulated, whereas miR-4284, miR-1202, miR-135a, miR-1973, and miR-204 were down-regulated compared with normal renal parenchyma. To identify consensus of differentially regulated miRNA between CCPRCC, CCRCC, and PRCC, we additionally determined differential miRNA expression using 2 publically available microarray data sets from the NCBI Gene Expression Omnibus database (GSE41282 and GSE3798). This comparison revealed that the miRNA expression profile of CCPRCC shows some overlapping characteristics between CCRCC and PRCC. Moreover, CCPRCC lacks dysregulation of important miRNAs typically associated with aggressive behavior. In summary, we describe the miRNA expression profile of a relatively infrequent type of renal cancer. Our results may help in understanding the molecular underpinning of this newly recognized entity.
登录
查看更多内容
DOI：
10.4161/cc.9.6.11006
发表时间：
2010-03-15
期刊：
Cell cycle (Georgetown, Tex.)
影响因子：
--
作者：
Chan SY;Loscalzo J
通讯作者：
Loscalzo J
影响因子：
--
作者：
Liu H;Brannon AR;Reddy AR;Alexe G;Seiler MW;Arreola A;Oza JH;Yao M;Juan D;Liou LS;Ganesan S;Levine AJ;Rathmell WK;Bhanot GV
通讯作者：
Bhanot GV
影响因子：
5.6
作者：
Tickoo, SK;dePeralta-Venturina, MN;Amin, MB
通讯作者：
Amin, MB
影响因子：
2.8
作者：
Ross, Ashley E.;Marchionni, Luigi;Schaeffer, Edward M.
通讯作者：
Schaeffer, Edward M.
影响因子：
7.5
作者：
Brunelli, Matteo;Eccher, Albino;Martignoni, Guido
通讯作者：
Martignoni, Guido