Genetic diversity and ex situ conservation of Loropetalum subcordatum, an endangered species endemic to China.
Genetic diversity and ex situ conservation of Loropetalum subcordatum, an endangered species endemic to China.
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DOI：
10.1186/s12863-018-0599-6
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发表时间：
2018-02-13
期刊：
影响因子：
2.9
通讯作者：
Huang JX
中科院分区：
文献类型：
--
作者：
Li BJ;Wang JY;Liu ZJ;Zhuang XY;Huang JX
红瓣木是我国特有的濒危植物，具有分布狭窄、种群规模小、受精延迟等特点。然而，整个现存的自然和迁地种群的遗传多样性尚未进行评估的日期。本研究对六个天然居群和一个迁地居群（目前已知的唯一一个迁地居群）的遗传多样性和遗传结构进行了分析。subcordatum）使用序列相关扩增多态性数据。采用15对引物组合进行聚合酶链反应，共扩增出553条可靠的DNA带，其中多态性带359条（63.28%）。居群内平均基因多样性较低，遗传分化程度较高。心下的Mantel检验表明，遗传距离与地理距离之间存在正相关关系，表明天然居群间显著的遗传分化可能是地理隔离的结果。此外，根据遗传结构模式，L。subcordatum分为三个集群。第1组为贵州荔波和广西环江的标本。第二组由Mt.广东五桂山。第3组由香港特别行政区的3个种群组成。此外，GD种群可能存在克隆繁殖。根据遗传信息分析和野外调查，迁地种群与其来源种群（GD）在遗传学上不匹配，其栖息地也与原自然栖息地不同。我们观察到，需要一些个别的GD种子，以提高ZS异位在未来。与以往基于SRAP的濒危植物研究相比，L。居群内平均基因多样性极低，居群间遗传分化较高。目前，由于采集的样本不具代表性，种群规模较小，以及人为改变了栖息地，独特的迁地种群尚未成功。建议潜在的策略，以提高该物种的保护。本文的在线版本（10.1186/s12863-018-0599-6）包含补充材料，可供授权用户使用。
Loropetalum subcordatum is an endangered species endemic to China that is characterized by narrow distribution, small population size, and delayed fertilization. However, the genetic diversity of the entire extant natural and ex situ populations has not been assessed to date. In this study, we evaluated the genetic diversity and structure of six natural populations and a single ex situ population (the only known ex situ population of L. subcordatum) using sequence-related amplified polymorphism data. In total, 553 reliable DNA bands, of which 359 (63.28%) were polymorphic, were amplified by polymerase chain reaction with combinations of 15 primers. Low average gene diversity within populations and high genetic differentiation were detected in L. subcordatum. A Mantel test demonstrated that there was a positive correlation between genetic and geographic distances, indicating that significant genetic divergence was likely the result of geographic isolation among natural populations. Furthermore, based on genetic structure patterns, populations of L. subcordatum were divided into three clusters. Group 1 was composed of specimens from Libo, Guizhou Province (GZ) and Huanjiang, Guangxi Zhuang Autonomous Region (GX). Group 2 was composed of Mt. Wuguishan, Guangdong Province (GD). Group 3 was composed of three populations in Hong Kong Special Administrative Region. Additionally, clonal reproduction probably existed in GD population. According to the genetic information analysis and field survey, the ex situ population did not match its source population (GD) in terms of genetics, and its habitat was different from the original natural habitat. We observed that a few individual GD seeds were needed to improve ZS ex situ in the future. Compared to previous SRAP-based studies of endangered plants, L. subcordatum had extremely low average gene diversity within populations and high genetic differentiation among populations. At present, the unique ex situ population has not been successful due to non-representative samples being taken, a smaller population size, and man-made changes in habitat. Potential strategies are suggested to improve the conservation of this species. The online version of this article (10.1186/s12863-018-0599-6) contains supplementary material, which is available to authorized users.
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