Altered antibody profiles against common infectious agents in chronic disease.
Altered antibody profiles against common infectious agents in chronic disease.
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DOI：
10.1371/journal.pone.0081635
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Iadarola MJ
中科院分区：
文献类型：
--
作者：
Burbelo PD;Ching KH;Morse CG;Alevizos I;Bayat A;Cohen JI;Ali MA;Kapoor A;Browne SK;Holland SM;Kovacs JA;Iadarola MJ
尽管评估对单个人类病原体的抗体应答具有重要的诊断价值，但针对多种感染因子的抗体谱尚未用于探索健康和疾病，主要是出于技术原因，我们假设感染和慢性疾病之间的相互作用可能通过分析针对多种因子的抗体来揭示。在此，使用定量荧光素酶免疫沉淀系统（LIPS）评估了来自三个疾病队列（包括具有致病性抗干扰素-γ自身抗体的患者）的针对一组13种常见感染因子的抗体水平（IFN-γ AAB），HIV和干燥综合征（SjS），以确定其抗体谱是否与对照受试者不同。与对照组相比，γ AAB患者表现出统计学上更高的抗VZV（p=0.0003）、EBV（p=0.002）、CMV（p=0.003）和C抗体水平。白色念珠菌（p=0.03），但脊髓灰质炎病毒抗体水平较低（p=0.04）。HIV患者与献血员对照组的比较显示，患者的CMV（p=0.0008）、HSV-2（p=0.0008）、EBV（p=0.001）和C.白色念珠菌（p=0.01），但显示出抗柯萨奇病毒B4（p=0.0008），脊髓灰质炎病毒（p=0.0005）， 和HHV-6 B（p=0.002）。最后，SjS患者的抗EBV抗体水平较高（p=0.03），但对几种肠道病毒的抗体水平较低，包括新鉴定的小核糖核酸病毒HCoSV-A（p=0.004），柯萨奇病毒B4（p=0.04）和脊髓灰质炎病毒（p=0.02）。对于IFN-γ AAB和HIV队列，主成分分析揭示了独特的抗体簇，显示出将患者与对照区分开的潜力。结果表明，针对这些和可能的其他常见感染因子的抗体谱可能有助于了解感染因子暴露、生态失调、适应性免疫和疾病活动之间的相互作用。
Despite the important diagnostic value of evaluating antibody responses to individual human pathogens, antibody profiles against multiple infectious agents have not been used to explore health and disease mainly for technical reasons. We hypothesized that the interplay between infection and chronic disease might be revealed by profiling antibodies against multiple agents. Here, the levels of antibodies against a panel of 13 common infectious agents were evaluated with the quantitative Luciferase Immunoprecipitation Systems (LIPS) in patients from three disease cohorts including those with pathogenic anti-interferon-γ autoantibodies (IFN-γ AAB), HIV and Sjögren’s syndrome (SjS) to determine if their antibody profiles differed from control subjects. The IFN-γ AAB patients compared to controls demonstrated statistically higher levels of antibodies against VZV (p=0.0003), EBV (p=0.002), CMV (p=0.003), and C. albicans (p=0.03), but lower antibody levels against poliovirus (p=0.04). Comparison of HIV patients with blood donor controls revealed that the patients had higher levels of antibodies against CMV (p=0.0008), HSV-2 (p=0.0008), EBV (p=0.001), and C. albicans (p=0.01), but showed decreased levels of antibodies against coxsackievirus B4 (p=0.0008), poliovirus (p=0.0005), and HHV-6B (p=0.002). Lastly, SjS patients had higher levels of anti-EBV antibodies (p=0.03), but lower antibody levels against several enteroviruses including a newly identified picornavirus, HCoSV-A (p=0.004), coxsackievirus B4 (p=0.04), and poliovirus (p=0.02). For the IFN-γ AAB and HIV cohorts, principal component analysis revealed unique antibody clusters that showed the potential to discriminate patients from controls. The results suggest that antibody profiles against these and likely other common infectious agents may yield insight into the interplay between exposure to infectious agents, dysbiosis, adaptive immunity and disease activity.
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作者：
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