In vitro susceptibility to rhinovirus infection is greater for bronchial than for nasal airway epithelial cells in human subjects.
In vitro susceptibility to rhinovirus infection is greater for bronchial than for nasal airway epithelial cells in human subjects.
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DOI：
10.1016/j.jaci.2009.03.010
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发表时间：
2009-06
影响因子：
14.2
通讯作者：
Avila, Pedro C.
中科院分区：
文献类型：
--
作者：
Lopez-Souza, Nilceia;Favoreto, Silvio;Wong, Hofer;Ward, Theresa;Yagi, Shigeo;Schnurr, David;Finkbeiner, Walter E.;Dolganov, Gregory M.;Widdicombe, Jonathan H.;Boushey, Homer A.;Avila, Pedro C.
Human rhinoviruses (HRVs) characteristically cause upper respiratory tract infection but they also infect the lower airways causing acute bronchitis and exacerbating asthma. Our purpose was to study ex-vivo the differences in the response to HRV infection of nasal and bronchial epithelial cultures from the same healthy and asthmatic individuals, using conditions favoring development of fully differentiated pseudostratified mucociliary epithelium. Cells from the inferior turbinates and bronchial tree of 5 healthy and 6 asthmatic individuals were cultured at an air-liquid interface. Cultures were infected with HRV-16 and after 48hrs the degree of infection was measured. Baseline median transepithelial resistance (Rte) was lower in human bronchial (HBE) than nasal (HNE) epithelial cell cultures (195Ω.cm2 [95%CI=164–252] vs 366Ω.cm2 [234–408] respectively, p<0.01). Virus replicated more easily in HBEs than HNEs based on virus shedding in apical wash (LogTCID50/0.1ml=2.0 [1.0–2.5] vs. 0.5 [0.5–1.5], p<0.01), and on a 20–30 fold greater viral load and number of infected cells in HBEs than in HNEs. The increases in expression of RANTES and protein kinase PKR were greater in HBE than in HNE cultures, as well as the concentrations of interleukin (IL)-8, IL-1α, RANTES and IP-10 in basolateral medium. However, no significant differences between asthmatic and healthy subjects (including interferon beta1 expression) were found. Differentiated nasal epithelial cells may have mechanisms of increased resistance to rhinovirus infection compared with bronchial epithelial cells. We could not confirm previous reports of increased susceptibility to HRV infection in epithelial cells from asthmatic subjects.
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影响因子：
10.5
作者：
Hunt, SL;Hsuan, JJ;Jackson, RJ
通讯作者：
Jackson, RJ
DOI：
10.1165/ajrcmb.10.2.8110476
发表时间：
1994-02-01
影响因子：
6.4
作者：
BARDIN, PG;JOHNSTON, SL;HOLGATE, ST
通讯作者：
HOLGATE, ST
影响因子：
24.3
作者：
Seemungal, TAR;Harper-Owen, R;Wedzicha, JA
通讯作者：
Wedzicha, JA
影响因子：
5.2
作者：
SMYTH, AR;SMYTH, RL;HEAF, DP
通讯作者：
HEAF, DP
哮喘支气管上皮细胞对鼻病毒感染的先天免疫反应不足。
DOI：
10.1084/jem.20041901
发表时间：
2005-03-21
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Wark PA;Johnston SL;Bucchieri F;Powell R;Puddicombe S;Laza-Stanca V;Holgate ST;Davies DE
通讯作者：
Davies DE