A supramolecular approach for preparation of size-controlled nanoparticles.
A supramolecular approach for preparation of size-controlled nanoparticles.
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DOI：
10.1002/anie.200900063
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发表时间：
2009
影响因子：
16.6
通讯作者：
Tseng, Hsian-Rong
中科院分区：
文献类型：
--
作者：
Wang, Hao;Wang, Shutao;Su, Helen;Chen, Kuan-Ju;Armijo, Amanda Lee;Lin, Wei-Yu;Wang, Yanju;Sun, Jing;Kamei, Ken-ichiro;Czernin, Johannes;Radu, Caius G.;Tseng, Hsian-Rong
在过去的几十年里，人们已经做出了巨大的努力来探索纳米颗粒在生物学和医学领域的应用。几种不同类型的纳米颗粒已经成功地进入了动物临床前研究，患者临床试验，甚至是常规临床实践中使用的成功商业产品。[1]例如，携带靶特异性配体的金纳米壳、[2]量子点[3，4]和超顺磁性纳米颗粒[5]已用于癌细胞的体内成像;药物分子已被包装到基于聚合物的纳米颗粒和/或脂质体中[6，7]以实现在疾病部位的受控释放;[8，9]和带正电荷的纳米颗粒已用作体外和体内遗传操作和编程的非病毒递送系统。[1，10，11]然而，仍然迫切需要开发新的合成方法来生产新一代纳米颗粒，其具有1）可控的尺寸和形态，2）低毒性，相容的免疫原性和体内降解性，以及3）适当的表面电荷和化学性质，以改善生理稳定性和更长的循环时间。此外，多个功能，[12]例如用于成像技术（即光学成像，磁共振成像（MRI）和正电子发射断层扫描（PET））实时监测的报告系统，用于疾病特异性递送的靶向配体，以及用于包装和释放药物和基因的可控机制，将被赋予单个纳米颗粒以并行进行多个应用。与能够形成/破坏共价键的常规化学合成不同，超分子化学结合了两个基本概念：自组装和分子识别。超分子化学提供了一个强大的和方便的方法来制备纳米结构材料的分子积木。[13-18]自组装的概念已被广泛用于制备有机纳米粒子。例如，使用磷脂的自组装制备的脂质体和纳米尺度囊泡[7]可以作为药物和基因递送的强大纳米载体;自组装的两亲性共聚物结构单元自发形成纳米颗粒，其可以用于药物递送和分子成像。[19-22]然而，很明显，“分子识别”的概念似乎是一个被低估的因素，这可能导致更复杂的合成方法，[23]允许精确控制所得纳米颗粒的性质。β-环糊精（CD）是生物医学应用中最常用的超分子结构单元之一。[24含CD的阳离子聚合物已被用作高效递送siRNA的载体。通过CD/金刚烷（Ad）识别，Ad-官能化的聚乙二醇（PEG）链被接枝到纳米颗粒上，以实现体内的长期体循环。[26]在此，我们报道了一种方便、灵活和模块化的合成方法（图1），用于制备尺寸可控的超分子纳米颗粒（SNPs）。利用CD/Ad识别技术实现了三种不同分子结构单元的SNPs自组装，即1）Ad接枝的第一代聚酰胺胺树状聚合物（n-Ad-PAMAM），2）β-CD接枝的支链聚乙烯亚胺（MW= 10 kD），CD-PEI，和3）Ad官能化的PEG化合物（MW= 5 kD），Ad-PEG。虽然类似于以前报道的“砖块和砂浆”策略[23]来构建交联网络，[27]我们设计的独特性...
Over the past decades, significant efforts have been devoted to explore the use of nanoparticles in the fields of biology and medicine. Several different types of nanoparticles have successfully made their way into preclinical studies in animals, clinic trials in patients, or even successful commercial products used in routine clinical practice.[1] For example, gold nanoshells,[2] quantum dots,[3, 4] and super-paramagnetic nanoparticles [5] that carry target-specific ligands have been employed for in vivo imaging of cancerous cells; drug molecules have been packaged into polymer-based nanoparticles and/or liposomes [6, 7] to achieve controlled release at the disease sites;[8, 9] and positively charged nanoparticles have served as nonviral delivery systems for both in vitro and in vivo genetic manipulation and programming.[1, 10, 11] However, there remains an imperious desire to develop novel synthetic approaches to produce a new generation of nanoparticles that have 1) controllable sizes and morphologies, 2) low toxicity, compatible immunogenicity and in vivo degradability, and 3) proper surface charges and chemistry for improved physiological stability and longer circulation time. Moreover, multiple functions,[12] such as reporter systems for real-time monitoring with imaging techniques (ie, optical imaging, magnetic resonance imaging (MRI), and positron emission tomography (PET)), targeting ligands for disease-specific delivery, and a controllable mechanism for packaging and releasing drugs and genes, will be conferred to individual nanoparticles for conducting multiple applications in parallel. Unlike conventional chemical synthesis, which is capable of forming/breaking covalent bonds, supramolecular chemistry combines two basic concepts: self assembly and molecular recognition. Supramolecular chemistry offers a powerful and convenient approach for the preparation of nanostructured materials from molecular building blocks.[13–18] The concept of self-assembly has been extensively used to prepare organic nanoparticles. For example, liposomes and nanoscaled vesicles [7] which were prepared using self-assembly of phospholipids can serve as powerful nanocarriers for drug and gene delivery; self-assembled amphiphilic copolymer building blocks spontaneously form nanoparticles, which can be utilized for drug delivery and molecular imaging.[19–22] However, it is apparent that the concept of “molecular recognition” seems to be an underappreciated factor, which could lead to much more sophisticated synthetic approaches,[23] allowing precise control over the properties of the resulting nanoparticles. β-Cyclodextrin (CD) is one of the most commonly used supramolecular building blocks for a diverse range of biomedical applications.[24, 25] CD-containing cationic polymers have been employed as vectors for highly efficient delivery of siRNA. Through CD/adamantane (Ad) recognition, Ad-functionalized polyethylene glycol (PEG) chains were grafted onto the nanoparticles to enable long-term systemic circulation in vivo.[26] Herein, we report a convenient, flexible, and modular synthetic approach (Figure1) for the preparation of sizecontrollable supramolecular nanoparticles (SNPs). CD/Ad recognition was employed to achieve self-assembly of SNPs from three different molecular building blocks, namely 1) Adgrafted first-generation polyamidoamine dendrimer, n-Ad-PAMAM, 2) β-CD-grafted branched polyethylenimine (MW= 10kD), CD-PEI, and 3) Ad-functionalized PEG compound (MW= 5kD), Ad-PEG. Although similar to a previously reported “bricks and mortar” strategy [23] to construct cross-linked network,[27] the uniqueness of our design …
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影响因子：
18.3
作者：
Gratton, Stephanie E. A.;Williams, Stuart S.;Napier, Mary E.;Pohlhaus, Patrick D.;Zhou, Zhilian;Wiles, Kenton B.;Maynor, Benjamin W.;Shen, Clifton;Olafsen, Tove;Samulski, Edward T.;Desimone, Joseph M.
通讯作者：
Desimone, Joseph M.
影响因子：
15
作者：
Bertin, PA;Gibbs, JM;Nguyen, ST
通讯作者：
Nguyen, ST
影响因子：
64.8
作者：
Boal, AK;Ilhan, F;Rotello, VM
通讯作者：
Rotello, VM
影响因子：
2.8
作者：
Loo, C;Lin, A;Drezek, R
通讯作者：
Drezek, R
影响因子：
46.9
作者：
Gao, XH;Cui, YY;Nie, SM
通讯作者：
Nie, SM