Monovalent and unpoised status of most genes in undifferentiated cell-enriched Drosophila testis.
Monovalent and unpoised status of most genes in undifferentiated cell-enriched Drosophila testis.
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DOI：
10.1186/gb-2010-11-4-r42
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发表时间：
2010
期刊：
影响因子：
12.3
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Gan Q;Schones DE;Ho Eun S;Wei G;Cui K;Zhao K;Chen X
在未分化的果蝇细胞中，分化相关基因具有单价而非二价组蛋白修饰，这与干细胞中的分化相关基因相反。越来越多的证据表明，干细胞通过独特的转录网络和染色质结构来维持其身份。相反的表观遗传修饰H3K27me3和H3K4me3已被提出用于标记干细胞、祖细胞和前体细胞中的分化相关基因。此外，许多分化相关基因通过在启动子区域招募主动RNA聚合酶II （Pol II）来维持在平衡状态，为分化时的谱系特异性表达做准备。以前的研究是用培养的哺乳动物胚胎干细胞进行的。在较小程度上，染色质结构已经在其他模式生物，如果蝇，开辟了遗传分析的新途径。在这里，我们使用了从果蝇的一袋大理石突变株中分离出来的睾丸，其中生殖细胞处于未分化状态。我们使用ChIP-seq技术研究这些睾丸未分化细胞的内源性染色质结构。我们整合了ChIP-seq和RNA-seq数据，后者测量数字转录组。我们的全基因组分析表明，未分化细胞中的大多数分化相关基因缺乏活性染色质标记和主动性Pol II；相反，它们要么与抑制性H3K27me3标记有关，要么与无法检测到的标记有关。我们的研究结果表明，在未分化细胞富集的果蝇睾丸中，大多数分化相关基因与单价修饰相关，而不是二价修饰，这是一种染色质特征，与哺乳动物干细胞或前体细胞的数据不同，这可能反映细胞类型特异性，物种特异性，或两者兼有。
In undifferentiated Drosophila cells, differentiation-associated genes have monovalent, not bivalent histone modifications, in contrast to differentiation-associated genes in stem cells. Increasing evidence demonstrates that stem cells maintain their identities by a unique transcription network and chromatin structure. Opposing epigenetic modifications H3K27me3 and H3K4me3 have been proposed to label differentiation-associated genes in stem cells, progenitor and precursor cells. In addition, many differentiation-associated genes are maintained at a poised status by recruitment of the initiative RNA Polymerase II (Pol II) at their promoter regions, in preparation for lineage-specific expression upon differentiation. Previous studies have been performed using cultured mammalian embryonic stem cells. To a lesser extent, chromatin structure has been delineated in other model organisms, such as Drosophila, to open new avenues for genetic analyses. Here we use testes isolated from a Drosophila bag of marbles mutant strain, from which germ cells are in their undifferentiated status. We use these testes to study the endogenous chromatin structure of undifferentiated cells using ChIP-seq. We integrate the ChIP-seq with RNA-seq data, which measures the digital transcriptome. Our genome-wide analyses indicate that most differentiation-associated genes in undifferentiated cells lack an active chromatin mark and initiative Pol II; instead, they are associated with either the repressive H3K27me3 mark or no detectable mark. Our results reveal that most of the differentiation-associated genes in undifferentiated-cell-enriched Drosophila testes are associated with monovalent but not bivalent modifications, a chromatin signature that is distinct from the data reported in mammalian stem or precursor cells, which may reflect cell type specificity, species specificity, or both.
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影响因子：
64.5
作者：
Guenther, Matthew G.;Levine, Stuart S.;Young, Richard A.
通讯作者：
Young, Richard A.
DOI：
10.1073/pnas.0910177106
发表时间：
2009-10-27
影响因子：
11.1
作者：
Adelman, Karen;Kennedy, Megan A.;Rogatsky, Inez
通讯作者：
Rogatsky, Inez
影响因子：
11.8
作者：
Akkers, Robert C.;van Heeringen, Simon J.;Jacobi, Ulrike G.;Janssen-Megens, Eva M.;Francoijs, Kees-Jan;Stunnenberg, Hendrik G.;Veenstra, Gert Jan C.
通讯作者：
Veenstra, Gert Jan C.
影响因子：
23.9
作者：
Cui K;Zang C;Roh TY;Schones DE;Childs RW;Peng W;Zhao K
通讯作者：
Zhao K
影响因子：
4
作者：
Barski, Artem;Zhao, Keji
通讯作者：
Zhao, Keji