Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease.
Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease.
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DOI：
10.1371/journal.pone.0095543
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Guo L;Han L;Yang L;Zeng H;Fan D;Zhu Y;Feng Y;Wang G;Peng C;Jiang X;Zhou D;Ni P;Liang C;Liu L;Wang J;Mao C;Fang X;Peng M;Huang J
无性真菌尖孢镰刀菌(Fusarium oxporum f.sp.)香蕉枯萎病(Musa spp.)是一种危害严重的病原菌。为了了解Foc致病性的分子基础，对两个Foc分离物的基因组和转录本进行了测序。基因组分析表明，1号生理小种和4号生理小种的基因组结构与尖孢镰刀菌高度同源。番茄菌株Fol4287。在两个Foc基因组中都发现了大量与毒力相关的基因，包括可能参与根附着、细胞降解、毒素解毒、运输、次生代谢产物生物合成和信号转导的基因。重要的是，相对于Foc小种1分离物(Foc1)，Foc Race 4分离物(Foc4)已经进化出一些扩展的转运蛋白和转录因子基因家族，用于运输毒素和营养物质，这可能有助于其适应寄主环境并促进对香蕉的致病。转录组分析表明，与营养生长期相比，在接种香蕉‘巴西’48h后，Foc1和Foc4的转录反应存在显著差异。特别值得注意的是，在Foc4中比在Foc1中上调了更多的毒力相关基因。有丝分裂原激活的蛋白激酶Fmk1介导的侵袭生长通路、FGA1介导的G蛋白信号通路和致病相关的双组分系统等信号通路在Foc4中被激活，而在Foc1中不被激活。总之，Foc1和Foc4在基因含量和转录反应上的这些差异可能解释了它们在感染香蕉品种‘巴西’期间毒力的差异。FOC基因组序列有助于我们确定香蕉维管束枯萎病发生发展的致病机制。这将促进我们开发有效的方法来管理香蕉的维管束枯萎病，包括提高香蕉的抗病能力。
The asexual fungus Fusarium oxysporum f. sp. cubense (Foc) causing vascular wilt disease is one of the most devastating pathogens of banana (Musa spp.). To understand the molecular underpinning of pathogenicity in Foc, the genomes and transcriptomes of two Foc isolates were sequenced. Genome analysis revealed that the genome structures of race 1 and race 4 isolates were highly syntenic with those of F. oxysporum f. sp. lycopersici strain Fol4287. A large number of putative virulence associated genes were identified in both Foc genomes, including genes putatively involved in root attachment, cell degradation, detoxification of toxin, transport, secondary metabolites biosynthesis and signal transductions. Importantly, relative to the Foc race 1 isolate (Foc1), the Foc race 4 isolate (Foc4) has evolved with some expanded gene families of transporters and transcription factors for transport of toxins and nutrients that may facilitate its ability to adapt to host environments and contribute to pathogenicity to banana. Transcriptome analysis disclosed a significant difference in transcriptional responses between Foc1 and Foc4 at 48 h post inoculation to the banana ‘Brazil’ in comparison with the vegetative growth stage. Of particular note, more virulence-associated genes were up regulated in Foc4 than in Foc1. Several signaling pathways like the mitogen-activated protein kinase Fmk1 mediated invasion growth pathway, the FGA1-mediated G protein signaling pathway and a pathogenicity associated two-component system were activated in Foc4 rather than in Foc1. Together, these differences in gene content and transcription response between Foc1 and Foc4 might account for variation in their virulence during infection of the banana variety ‘Brazil’. Foc genome sequences will facilitate us to identify pathogenicity mechanism involved in the banana vascular wilt disease development. These will thus advance us develop effective methods for managing the banana vascular wilt disease, including improvement of disease resistance in banana.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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