The role of peritoneal alternatively activated macrophages in the process of peritoneal fibrosis related to peritoneal dialysis.
The role of peritoneal alternatively activated macrophages in the process of peritoneal fibrosis related to peritoneal dialysis.
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DOI：
10.3390/ijms140510369
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发表时间：
2013-05-17
影响因子：
5.6
通讯作者：
Yu XQ
中科院分区：
文献类型：
--
作者：
Wang J;Jiang ZP;Su N;Fan JJ;Ruan YP;Peng WX;Li YF;Yu XQ
已证实M2参与组织重塑和纤维化的发生，但M2在腹膜透析（PD）相关腹膜纤维化中的作用尚未阐明。因此，本研究旨在评估M2与PD相关的腹膜纤维化之间的关系。本研究通过腹腔注射乳酸-4.25%透析液（100 mL/kg）诱导C57BL/6J小鼠腹膜纤维化28 d，并在第18天和第21天分别用脂质体包封氯膦酸钠（LC，巨噬细胞特异性清除剂）腹腔注射治疗腹膜纤维化小鼠模型。第29天处死所有动物。采用马松三色法对顶骨腹膜进行染色，采用Western blotting、免疫荧光和实时荧光定量PCR检测ⅰ型胶原（Col-I）、纤维连接蛋白、甘露糖受体（CD206）、转化生长因子β (TGF-β)、趋化因子受体7 （CCR7）、几丁质酶3样3 （m-1）和精氨酸酶1 （Arg-1）的表达。结果显示，腹腔纤维化小鼠腹膜厚度、col -1、纤维连接蛋白、CD206、TGF-β、y -1、Arg-1等指标在腹腔纤维化小鼠模型中上调，LC组均下调。CCR7水平在模型组与治疗组间无显著差异。我们的研究表明，腹膜M2在PD相关腹膜纤维化过程中发挥了重要作用，可能是腹膜纤维化干预治疗的潜在靶点。
It has been confirmed that alternatively activated macrophages (M2) participate in tissue remodeling and fibrosis occurrence, but the effect of M2 on peritoneal fibrosis related to peritoneal dialysis (PD) hasn’t been elucidated. This study was therefore conducted to assess the association between M2 and peritoneal fibrosis related to PD. In this study, peritoneal fibrosis was induced by intraperitoneal (i.p.) injection of Lactate-4.25% dialysate (100 mL/kg) to C57BL/6J mice for 28 days, and liposome-encapsulated clodronate (LC, the specific scavenger of macrophages) was used to treat the peritoneal fibrosis mice model by i.p. injection at day 18 and day 21. All animals were sacrificed at day 29. Parietal peritonea were stained with Masson’s trichrome, and the expression of type I collagen (Col-I), fibronectin, mannose receptor (CD206), transforming growth factor beta (TGF-β), chemokine receptor 7 (CCR7), chitinase 3-like 3 (Ym-1) and arginase-1 (Arg-1) was determined by Western blotting, immunofluorescence and quantitative real-time PCR. Our results revealed that peritoneal thickness, Col-I, fibronectin, CD206, TGF-β, Ym-1 and Arg-1 were upregulated in the peritoneal fibrosis mice model, and all of these indexes were downregulated in those treated with LC. Additionally, there was no difference in the level of CCR7 between the model and treatment group. Our study indicated that peritoneal M2 played an important role in the process of peritoneal fibrosis related to PD and might be a potential target for intervention therapy of peritoneal fibrosis.
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影响因子：
3.7
作者：
Murray LA;Rosada R;Moreira AP;Joshi A;Kramer MS;Hesson DP;Argentieri RL;Mathai S;Gulati M;Herzog EL;Hogaboam CM
通讯作者：
Hogaboam CM
影响因子：
4.4
作者：
Martinez, Fernando O.;Gordon, Siamon;Mantovani, Alberto
通讯作者：
Mantovani, Alberto
DOI：
10.1084/jem.176.1.287
发表时间：
1992-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Stein M;Keshav S;Harris N;Gordon S
通讯作者：
Gordon S
影响因子：
3.6
作者：
BIEWENGA, J;VANDERENDE, MB;VANROOIJEN, N
通讯作者：
VANROOIJEN, N
影响因子：
6.1
作者：
Bellon, Teresa;Martinez, Virginia;Auxiliadora Bajo, Maria
通讯作者：
Auxiliadora Bajo, Maria