Phosphoproteomic Analysis of Thermomorphogenic Responses in Arabidopsis.
Phosphoproteomic Analysis of Thermomorphogenic Responses in Arabidopsis.
复制标题
拟南芥热形态发生反应的磷酸化蛋白质组学分析
DOI：
10.3389/fpls.2021.753148
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发表时间：
2021
影响因子：
5.6
通讯作者：
Liu JX
中科院分区：
文献类型：
--
作者：
Shao YJ;Zhu QY;Yao ZW;Liu JX
植物通过调整其生长和发育程序迅速适应环境温度升高。到目前为止，已经进行了一些实验来了解植物如何感知和应对温暖的温度。然而，温暖的温度信号如何从热传感器传递到转录调节因子在很大程度上是未知的。为了鉴定植物热响应性的新的早期调节剂，我们使用TMT（串联质量标签）标记和磷酸肽富集对经过或不经过3 h温暖温度（29°C）处理的拟南芥黄化幼苗进行磷酸蛋白质组学分析。总的来说，我们在5，125种蛋白质中鉴定了13，160种磷酸肽，具有10，700个可量化的磷酸化位点。其中，200个位点（180个蛋白质）被上调，而120个位点（87个蛋白质）被高温下调。GO（基因本体论）分析表明，磷酸化相关的分子功能的差异磷酸化的蛋白质富集。我们从它们中选择了ATL 6（ARABIDOPSIS TOXICOS EN LEVADURA 6），并在拟南芥中表达了其天然和磷酸化位点突变（S343 A S357 A）形式，发现ATL 6的突变形式在体内和无细胞降解测定中均不如天然形式稳定。总之，我们的数据揭示了广泛的蛋白质磷酸化过程中的热响应，提供了新的候选蛋白/基因，为研究植物热形态建成在未来。
Plants rapidly adapt to elevated ambient temperature by adjusting their growth and developmental programs. To date, a number of experiments have been carried out to understand how plants sense and respond to warm temperatures. However, how warm temperature signals are relayed from thermosensors to transcriptional regulators is largely unknown. To identify new early regulators of plant thermo-responsiveness, we performed phosphoproteomic analysis using TMT (Tandem Mass Tags) labeling and phosphopeptide enrichment with Arabidopsis etiolated seedlings treated with or without 3h of warm temperatures (29°C). In total, we identified 13,160 phosphopeptides in 5,125 proteins with 10,700 quantifiable phosphorylation sites. Among them, 200 sites (180 proteins) were upregulated, while 120 sites (87 proteins) were downregulated by elevated temperature. GO (Gene Ontology) analysis indicated that phosphorelay-related molecular function was enriched among the differentially phosphorylated proteins. We selected ATL6 (ARABIDOPSIS TOXICOS EN LEVADURA 6) from them and expressed its native and phosphorylation-site mutated (S343A S357A) forms in Arabidopsis and found that the mutated form of ATL6 was less stable than that of the native form both in vivo and in cell-free degradation assays. Taken together, our data revealed extensive protein phosphorylation during thermo-responsiveness, providing new candidate proteins/genes for studying plant thermomorphogenesis in the future.
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影响因子：
16.6
作者：
Vu LD;Xu X;Zhu T;Pan L;van Zanten M;de Jong D;Wang Y;Vanremoortele T;Locke AM;van de Cotte B;De Winne N;Stes E;Russinova E;De Jaeger G;Van Damme D;Uauy C;Gevaert K;De Smet I
通讯作者：
De Smet I
影响因子：
64.8
作者：
Jung, Jae-Hoon;Barbosa, Antonio D.;Wigge, Philip A.
通讯作者：
Wigge, Philip A.
影响因子：
4.6
作者：
Furutani, Masahiko;Sakamoto, Norihito;Tasaka, Masao
通讯作者：
Tasaka, Masao
影响因子：
8.8
作者：
Ding, Lan;Wang, Shuo;Liu, Jian-Xiang
通讯作者：
Liu, Jian-Xiang
影响因子：
7.2
作者：
Chen, Mo-Xian;Zhang, Kai-Lu;Fang, Yan-Ming
通讯作者：
Fang, Yan-Ming