Molecular cloning from neurulating Ambystoma mexicanum embryos of the cDNA encoding an orphan nuclear receptor (aDOR1) closely related to TR2-11.
Molecular cloning from neurulating Ambystoma mexicanum embryos of the cDNA encoding an orphan nuclear receptor (aDOR1) closely related to TR2-11.
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从墨西哥钝口螈胚胎中分子克隆编码与 TR2-11 密切相关的孤儿核受体 (aDOR1) 的 cDNA。
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
C. Séguin
中科院分区：
文献类型：
--
作者：
L. Wirtanen;V. Huard;C. Séguin
我们已经分离出一个cDNA编码一种新的孤儿核受体，aDOR 1，密切相关的睾丸受体-2（TR 2）孤儿受体家族成员，从神经元发育的Ambystoma mexicanum胚胎。该cDNA序列预测了416个氨基酸的蛋白质一级序列，计算的分子量为45.8 kDa。虽然aDOR 1和hTR 2 -11的DNA结合结构域共享96%的同一性，但在肽的两端观察到相当大的差异。在N-末端，aDOR 1与hTR 2 -11有66%的同一性，并且比hTR 2 - 11长37个氨基酸。在C-末端，尽管有更大的相似性（69%），aDOR 1比hTR 2亚型短得多，似乎编码一个独特的配体结合结构域。逆转录聚合酶链反应（RT-PCR）检测aDOR 1的表达。在卵子发生过程中检测到高水平的mRNA，它们在卵裂阶段保持高水平，并在中期囊胚过渡（MBT）下降。转录本在原肠胚形成结束时再次增加，在神经胚形成期间达到峰值水平，并在神经管关闭后趋于平稳。在神经元解剖沿着前后轴，aDOR 1 mRNA富集在胚胎的两端，而没有特定的分布是有利的沿着背腹轴。在原肠胚形成开始时，视黄酸（RA）处理不影响神经胚中的总体mRNA水平，也不影响其沿沿着两个轴的分布。在成人中，表达主要在脑中;除肌肉外，在所有胚层衍生物中均可检测到较低水平（约15%）。这些结果表明，aDOR 1可能需要在发育的卵裂阶段发生的早期决定事件，并可能参与胚胎发生和脑功能。
We have isolated a cDNA encoding a novel orphan nuclear receptor, aDOR1, closely related to testicular receptor-2 (TR2) orphan receptor family members, from neurulating Ambystoma mexicanum embryos. The cDNA sequence predicts a protein primary sequence of 416 amino acids with a calculated molecular weight of 45.8 kDa. While the DNA-binding domains of aDOR1 and hTR2-11 share 96% identity, considerable divergence is observed at both extremities of the peptides. At the N-terminus, aDOR1 is 66% identical to hTR2-11 and longer by 37 amino acids. At the C-terminus, despite a greater similarity (69%), aDOR1 is much shorter than the hTR2 isoforms and seems to encode a distinct ligand-binding domain. Expression of aDOR1 was studied by the reverse transcription polymerase chain reaction assay (RT-PCR). High mRNA levels were detected during oogenesis, they remained high during the cleavage stage, and decreased at the mid-blastula transition (MBT). Transcripts increased again at the end of gastrulation, reached a peak level during neurulation, and leveled off after closure of the neural tube. In neurulas dissected along the anteroposterior axis, aDOR1 mRNA was enriched at both extremities of the embryo, while no particular distribution was favored along the dorsoventral axis. Retinoic acid (RA) treatments at the beginning of gastrulation did not affect overall mRNA levels in the neurula nor its distribution along both axes. In the adult, expression was predominant in the brain; lower levels (about 15%) were detectable in all germ layer derivatives, except muscle. These results suggest that aDOR1 may be required for the early determination events occurring during the cleavage stages of development, and may be involved in embryogenesis and in brain function.
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DOI：
10.1073/pnas.91.13.6040
发表时间：
1994-06-21
影响因子：
11.1
作者：
CHANG, CS;DASILVA, SL;BURBACH, JPH
通讯作者：
BURBACH, JPH
影响因子：
4.6
作者：
C. Mendelsohn;D. Lohnes;D. Décimo;T. Lufkin;M. Lemeur;P. Chambon;M. Mark
通讯作者：
C. Mendelsohn;D. Lohnes;D. Décimo;T. Lufkin;M. Lemeur;P. Chambon;M. Mark
影响因子：
--
作者：
Fang Chen;A. J. Cooney;Yaolin Wang;Simon W. Law;B. W. O'malley
通讯作者：
Fang Chen;A. J. Cooney;Yaolin Wang;Simon W. Law;B. W. O'malley
一种由选择性剪接母体 RNA 编码的新型海胆核受体。
DOI：
10.1006/dbio.1996.0171
发表时间：
1996
期刊：
Developmental biology.
影响因子：
--
作者：
Kontrogianni-Konstantopoulos,A;Vlahou,A;Vu,D;Flytzanis,CN
通讯作者：
Flytzanis,CN
影响因子：
10.5
作者：
CHEN, WS;MANOVA, K;DARNELL, JE
通讯作者：
DARNELL, JE