Synergism of BSF-2/interleukin 6 and interleukin 3 on development of multipotential hemopoietic progenitors in serum-free culture.
Synergism of BSF-2/interleukin 6 and interleukin 3 on development of multipotential hemopoietic progenitors in serum-free culture.
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DOI：
10.1084/jem.168.3.879
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发表时间：
1988-09-01
影响因子：
15.3
通讯作者：
AKABANE, T
中科院分区：
文献类型：
--
作者：
KOIKE, K;NAKAHATA, T;TAKAGI, M;KOBAYASHI, T;ISHIGURO, A;TSUJI, K;NAGANUMA, K;OKANO, A;AKIYAMA, Y;AKABANE, T
我们使用含血清培养物和无血清培养物研究了 B 细胞刺激因子 2/白细胞介素 6 (BSF-2/IL-6) 对小鼠造血祖细胞发育的影响。在含血清培养物中，BSF-2 主要支持正常小鼠和 5-氟尿嘧啶 (5-FU) 治疗小鼠脾细胞的多能母细胞集落。在无血清培养中，在 BSF-2 存在的情况下未观察到集落生长。在含有 IL-3 的无血清培养物中添加 BSF-2 导致 5-FU 处理小鼠的脾细胞和骨髓细胞中多能祖细胞形成的集落数量显着增加，而对正常小鼠脾细胞形成的单系或寡系集落数量没有影响。这些结果表明BSF-2和IL-3对多能祖细胞有协同作用，但对成熟祖细胞没有协同作用。当BSF-2添加到含有低浓度IL-3（1 U/ml、4 U/ml）的培养物中时，对集落形成几乎没有影响，5-FU处理的小鼠的脾细胞和骨髓细胞形成的集落总数显着增加。 BSF-2 和 40 U/ml IL-3 的组合导致 GMM 集落显着增大。因此，BSF-2似乎增强了多能造血祖细胞对IL-3的敏感性。
We investigated the effects of B cell stimulatory factor 2/interleukin 6 (BSF-2/IL-6) on the development of murine hemopoietic progenitors using serum-containing culture and serum-free culture. In serum- containing culture, BSF-2 mainly supported multipotential blast cell colonies from spleen cells of normal and 5-fluorouracil (5-FU)-treated mice. In serum-free culture, no colony growth was seen in the presence of BSF-2. Addition of BSF-2 to the serum-free culture containing IL-3 resulted in a significant increase in the number of colonies formed from multipotential progenitors in spleen cells and bone marrow cells of 5-FU-treated mice, whereas no effects were seen on the number of single or oligolineage colonies formed by the spleen cells of normal mice. These results suggested that BSF-2 and IL-3 act synergistically on the multipotential progenitors but not on the maturer progenitors. When BSF-2 was added to a culture containing low concentrations of IL-3 (1 U/ml, 4 U/ml), which had little effect on colony formation, the number of total colonies formed by the spleen cells and bone marrow cells of 5-FU-treated mice increased significantly. The combination of BSF-2 and 40 U/ml of IL-3 resulted in a significant enlargement of GMM colonies. Thus, BSF-2 appears to enhance the sensitivity of multipotential hemopoietic progenitors to IL-3.
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影响因子：
56.9
作者：
METCALF, D
通讯作者：
METCALF, D
影响因子：
5.6
作者：
NAKAHATA, T;OGAWA, M
通讯作者：
OGAWA, M
影响因子：
64.8
作者：
HIRANO, T;YASUKAWA, K;KISHIMOTO, T
通讯作者：
KISHIMOTO, T
DOI：
10.1073/pnas.84.24.9035
发表时间：
1987-12-01
影响因子：
11.1
作者：
IKEBUCHI, K;WONG, GG;OGAWA, M
通讯作者：
OGAWA, M
影响因子：
5.6
作者：
SUDA, T;SUDA, J;IHLE, JN
通讯作者：
IHLE, JN