Iron and ferritin accumulate in separate cellular locations in Phaseolus seeds.
Iron and ferritin accumulate in separate cellular locations in Phaseolus seeds.
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DOI：
10.1186/1471-2229-10-26
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发表时间：
2010-02-11
影响因子：
5.3
通讯作者：
Stougaard J
中科院分区：
文献类型：
--
作者：
Cvitanich C;Przybyłowicz WJ;Urbanski DF;Jurkiewicz AM;Mesjasz-Przybyłowicz J;Blair MW;Astudillo C;Jensen EØ;Stougaard J
铁是所有生物体内重要的微量营养素。世界上近25%的人口缺铁，缺铁是贫血的主要原因。在植物中，缺铁会导致黄化和产量降低。当动物和植物的饮食或环境缺乏生物可利用的铁时，它们都可能缺铁。减少人类铁营养不良的可持续方法是开发具有更高生物可利用铁含量的主要作物。了解铁在作物种子中的积累位置和方式将增加对植物铁代谢的了解，并将有助于增加生物可利用铁的主食生产。本研究揭示了三种菜豆属植物种子中铁元素的分布，包括13个基因型的P. vulgaris、P. coccineus和P. lunatus。我们发现高浓度的铁积聚在普通假单胞菌和球菌假单胞菌种子血管前组织周围的细胞中。使用珀尔斯普鲁士蓝法，我们能够在表皮细胞、表皮附近细胞和血管前组织周围细胞的细胞质中检测到铁。相比之下，豆科植物中作为主要铁储存蛋白的铁蛋白仅在种子胚的淀粉质体中检测到。利用非破坏性的微粒子诱导x射线发射（pixe）技术，研究人员发现，根据基因型的不同，靠近维管束的组织含有高达500 μg -1的铁。与寻常假单胞菌和尾尾假单胞菌相比，我们没有观察到月亮假单胞菌子叶维管前组织周围细胞中的铁积累。将安第斯基因型与中美洲基因型杂交，培育出一种种皮和子叶均含高浓度铁的新型富铁基因型NUA35。这些结果强调了模式生物研究与作物分析相辅相成的重要性，并建议将铁的分布标准纳入豆类生物强化的选择策略中。
Iron is an important micronutrient for all living organisms. Almost 25% of the world population is affected by iron deficiency, a leading cause of anemia. In plants, iron deficiency leads to chlorosis and reduced yield. Both animals and plants may suffer from iron deficiency when their diet or environment lacks bioavailable iron. A sustainable way to reduce iron malnutrition in humans is to develop staple crops with increased content of bioavailable iron. Knowledge of where and how iron accumulates in seeds of crop plants will increase the understanding of plant iron metabolism and will assist in the production of staples with increased bioavailable iron. Here we reveal the distribution of iron in seeds of three Phaseolus species including thirteen genotypes of P. vulgaris, P. coccineus, and P. lunatus. We showed that high concentrations of iron accumulate in cells surrounding the provascular tissue of P. vulgaris and P. coccineus seeds. Using the Perls' Prussian blue method, we were able to detect iron in the cytoplasm of epidermal cells, cells near the epidermis, and cells surrounding the provascular tissue. In contrast, the protein ferritin that has been suggested as the major iron storage protein in legumes was only detected in the amyloplasts of the seed embryo. Using the non-destructive micro-PIXE (Particle Induced X-ray Emission) technique we show that the tissue in the proximity of the provascular bundles holds up to 500 μg g-1 of iron, depending on the genotype. In contrast to P. vulgaris and P. coccineus, we did not observe iron accumulation in the cells surrounding the provascular tissues of P. lunatus cotyledons. A novel iron-rich genotype, NUA35, with a high concentration of iron both in the seed coat and cotyledons was bred from a cross between an Andean and a Mesoamerican genotype. The presented results emphasize the importance of complementing research in model organisms with analysis in crop plants and they suggest that iron distribution criteria should be integrated into selection strategies for bean biofortification.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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