A specific type of Argonaute phosphorylation regulates binding to microRNAs during C. elegans development.
A specific type of Argonaute phosphorylation regulates binding to microRNAs during C. elegans development.
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DOI：
10.1016/j.celrep.2022.111822
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发表时间：
2022-12-13
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
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Argonaute proteins are at the core of the microRNA-mediated gene silencing pathway essential for animals. In C. elegans, the microRNA-specific Argonautes ALG-1 and ALG-2 regulate multiple processes required for proper animal developmental timing and viability. Here, we identified a phosphorylation site on ALG-1 that modulates microRNA association. Mutating ALG-1 serine 642 into a phospho-mimicking residue impairs microRNA binding and causes embryonic lethality and post-embryonic phenotypes that are consistent with alteration of microRNA functions. Monitoring microRNA levels in alg-1 phosphorylation mutant animals reveal that microRNA passenger strands increase in abundance but are not preferentially loaded into ALG-1, indicating that the miRNA binding defects could lead to microRNA duplex accumulation. Our genetic and biochemical experiments support the protein kinase A (PKA) KIN-1 as the putative kinase that phosphorylates ALG-1 serine 642. Altogether, our data indicate that PKA triggers the ALG-1 phosphorylation to regulate its microRNAs association during C. elegans development.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
56.9
作者：
Boehm, M;Slack, F
通讯作者：
Slack, F
影响因子：
64.5
作者：
Elkayam E;Kuhn CD;Tocilj A;Haase AD;Greene EM;Hannon GJ;Joshua-Tor L
通讯作者：
Joshua-Tor L
影响因子：
64.5
作者：
Diederichs, Sven;Haber, Daniel A.
通讯作者：
Haber, Daniel A.
DOI：
10.1126/science.1164899
发表时间：
2009-04-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bethke A;Fielenbach N;Wang Z;Mangelsdorf DJ;Antebi A
通讯作者：
Antebi A