Suspended Collagen Hydrogels to Replicate Human Colonic Epithelial Cell Interactions with Immune Cells.
Suspended Collagen Hydrogels to Replicate Human Colonic Epithelial Cell Interactions with Immune Cells.
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DOI：
10.1002/adbi.202200129
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发表时间：
2022-11
期刊：
影响因子：
3.7
通讯作者：
Allbritton, Nancy L.
中科院分区：
文献类型：
--
作者：
Hinman, Samuel S.;Massaro, Angelo;Wang, Yuli;Sims, Christopher E.;Kim, Raehyun;Allbritton, Nancy L.
人类结肠在液体和盐的吸收中起着关键作用，并拥有最大的免疫区室。人们广泛需要具有先天免疫系统的人结肠生理学的体外模型。描述了一种生产具有支撑悬浮的、非化学交联的胶原水凝胶支架的支柱网络的盒的方法，所述支架与原代胃肠上皮和迁移性炎性细胞的共培养物相容。在悬浮胶原上培养的上皮单层具有与体内存在的类似的极化和分化的细胞群体。该上皮层显示出适当的屏障功能，TEER ≥ 1，500 Ω cm 2，表观渗透率≤10−5 cm 2 s−1。接种在支架底面上的免疫细胞在24小时内迁移到上皮层，以响应艰难梭菌毒素A的管腔刺激诱导的IL-8的上皮产生。这些研究表明，该体外平台具有功能性原代结肠上皮层，其具有能够响应于来自上皮的促炎线索而募集的免疫细胞区室。原代人结肠上皮层形成于悬浮在聚酯支柱框架上的非化学交联胶原水凝胶上。该水凝胶支持免疫细胞区室，该免疫细胞区室能够通过迁移通过水凝胶以与上皮细胞接触来响应来自上皮的促炎线索。该平台能够研究人类结肠上皮和肠道免疫区室界面。
The human colon plays critical roles in fluid and salt absorption and harbors the largest immune compartment. There is widespread need for in vitro models of human colon physiology with its innate immune system. A method is described to produce a cassette with a network of struts supporting a suspended, non-chemically crosslinked collagen hydrogel scaffold compatible with the co-culture of primary gastrointestinal epithelium and migratory inflammatory cells. The epithelial monolayer cultured on the suspended collagen possessed a population of polarized and differentiated cells similar to that present in vivo. This epithelial layer displayed proper barrier function with a TEER ≥1,500 Ω cm2 and an apparent permeability ≤10−5 cm2 s−1. Immune cells plated on the basal face of the scaffold transmigrated over a period of 24 h to the epithelial layer in response to epithelial production of IL-8 induced by luminal stimulation of Clostridium difficile Toxin A. These studies demonstrate that this in vitro platform possesses a functional primary colonic epithelial layer with an immune cell compartment capable of recruitment in response to pro-inflammatory cues coming from the epithelium. A primary human colonic epithelial layer was formed on a non-chemically crosslinked collagen hydrogel suspended on a framework of polyester struts. The hydrogel supported an immune cell compartment capable of responding to pro-inflammatory cues from the epithelium by migration through the hydrogel to interface with the epithelial cells. This platform enables studies of the human colonic epithelium and intestinal immune compartment interface.
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影响因子：
2.4
作者：
Bischoff SC;Barbara G;Buurman W;Ockhuizen T;Schulzke JD;Serino M;Tilg H;Watson A;Wells JM
通讯作者：
Wells JM
影响因子：
14.8
作者：
Dekkers, Johanna F.;Alieva, Maria;Rios, Anne C.
通讯作者：
Rios, Anne C.
DOI：
10.1073/pnas.75.5.2458
发表时间：
1978-01-01
影响因子：
11.1
作者：
COLLINS, SJ;RUSCETTI, FW;GALLO, RC
通讯作者：
GALLO, RC
影响因子：
5.8
作者：
Adatia, K.;Raja, M.;Vadgama, P.
通讯作者：
Vadgama, P.
影响因子：
35.7
作者：
Alatab, Sudabeh;Sepanlou, Sadaf G.;Naghavi, Mohsen
通讯作者：
Naghavi, Mohsen