Molecular Epidemiology and Clinical Features of Enteroviruses-Associated Hand, Foot, and Mouth Disease and Herpangina Outbreak in Zunyi, China, 2019.
Molecular Epidemiology and Clinical Features of Enteroviruses-Associated Hand, Foot, and Mouth Disease and Herpangina Outbreak in Zunyi, China, 2019.
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2019年遵义市肠道病毒相关手足口病及疱疹样咽峡炎暴发的分子流行病学及临床特征
DOI：
10.3389/fmed.2021.656699
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发表时间：
2021
影响因子：
3.9
通讯作者：
Wu K
中科院分区：
文献类型：
--
作者：
Ai Y;Zhang W;Wu J;Zhang J;Shen M;Yao S;Deng C;Li X;Wu D;Tian P;Cheng X;Zha H;Wu K
背景资料：手足口病和疱疹性咽峡炎是两种最常见的儿童传染病，均与肠道病毒感染有关。本研究的目的是确定2019年中国遵义市引起手足口病/HA的肠道病毒的分子流行病学，并描述病例的临床特征。研究方法：我们收集了2019年3月1日至7月31日期间遵义医学院第三附属医院实验室确诊的EV相关手足口病/HA病例的人口统计学和临床特征、实验室数据等信息。采用一步真实的时间RT-PCR或部分VP 1基因测序和序列比对确定EV类型。根据CVA 6、CVA 2和CVA 5的部分VP 1基因序列，采用邻接法进行系统发育分析。比较了感染最流行EV类型患者的临床特征和实验室结果的差异。结果：从2019年3月1日至7月31日，确诊了1，377例EV相关的手足口病/HA住院患者。其中EV-A71相关4例（0.3%，4/1,377），CVA 16相关84例（6.1%，84/1,377），非EV-A71/CVA 16相关1,289例（93.6%，1,289/1,377）。在随机抽取的372例非EV-A71/CVA 16病例中，273例已成功确定EV类型，其中手足口病166例，HA 107例。在手足口病病例中，三种最常见的类型是CVA 6（80.7%，134/166），CVA2（5.4%，9/166）和CVA 5同样，对于HA病例，三种最流行的血清型为CVA 6（36.5%，39/107）、CVA 2（21.5%，23/107）和CVA 5（18.7%，20/107）。系统发育分析表明，2019年遵义暴发期间，CVA 5的D亚支、CVA 6和CVA 2的E亚支占优势。与CVA 16相比，CVA 2、CVA 5、CVA 6引起的高热和严重感染的发生率更高。结论：遵义市近期手足口病/HA暴发是由于CVA 6、CVA 2和CVA 5的较高发病率所致。针对这些类型的新型诊断试剂和疫苗对于监测和控制EV感染将是重要的。
Background: Hand, foot and mouth disease (HFMD) and herpangina (HA), two of the most common childhood infectious diseases, are associated with enteroviruses (EVs) infection. The aim of this study was to identify the molecular epidemiology of enterovirus causing HFMD/HA in Zunyi, China, during 2019, and to describe the clinical features of the cases. Methods: We collected the information on demographic and clinical characteristics, laboratory data of laboratory-confirmed EVs associated HFMD/HA cases in Zunyi Medical University Third Affiliated Hospital between March 1 and July 31, 2019. EV types were determined by either one-step real time RT-PCR or partial VP1 gene sequencing and sequence alignment. Phylogenetic analysis of CVA6, CVA2, and CVA5 were established based on the partial VP1 gene sequences by neighbor-joining method. Differences in clinical characteristics and laboratory results of the cases were compared among patients infected with the most prevalent EV types. Results: From 1 March to 31 July 2019, 1,377 EVs associated HFMD/HA inpatients were confirmed. Of them, 4 (0.3%, 4/1,377) were EV-A71-associated cases, 84 (6.1%, 84/1,377) were CVA16-associated cases, and 1,289 (93.6%, 1,289/1,377) were non-EV-A71/CVA16-associated cases. Of the randomly selected 372 non-EV-A71/CVA16 cases, EV types have been successfully determined in 273 cases including 166 HFMD and 107 HA cases. For HFMD cases, the three most common types were CVA6 (80.7%, 134/166), CVA2 (5.4%, 9/166) and CVA5 (3.0%, 5/166); similarly, for HA cases, the three most prevalent serotypes were CVA6 (36.5%, 39/107), CVA2 (21.5%, 23/107) and CVA5 (18.7%, 20/107). Phylogenetic analysis showed that subclade D of CVA5, and subclade E of CVA6 and CVA2 were predominant in Zunyi during the outbreak in 2019. Compared with the cases caused by CVA16, the incidence of high fever and severe infection associated with CVA2, CVA5, and CVA6 was higher. Conclusions: The recent HFMD/HA outbreak in Zunyi is due to a larger incidence of CVA6, CVA2, and CVA5. Novel diagnostic reagents and vaccines against these types would be important to monitor and control EV infections.
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影响因子：
11.8
作者：
Li Y;Chang Z;Wu P;Liao Q;Liu F;Zheng Y;Luo L;Zhou Y;Chen Q;Yu S;Guo C;Chen Z;Long L;Zhao S;Yang B;Yu H;Cowling BJ
通讯作者：
Cowling BJ
影响因子：
1.6
作者：
Zhang W;Huang B;She C;Liu Y;Tong H;Wang F;Wu K
通讯作者：
Wu K
引起单纯性手足口病的肠道病毒血清型谱及不同临床样本的肠道病毒诊断率
影响因子：
11.8
作者：
Gao, Lidong;Zou, Gang;Yu, Hongjie
通讯作者：
Yu, Hongjie
影响因子：
3.6
作者：
Hu, Ya-Qian;Xie, Guang-Cheng;Duan, Zhao-Jun
通讯作者：
Duan, Zhao-Jun
DOI：
10.12659/msm.901364
发表时间：
2017-05-25
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Weng Y;Chen W;He W;Huang M;Zhu Y;Yan Y
通讯作者：
Yan Y