Astaxanthin prevents ischemia-reperfusion injury of the steatotic liver in mice.
Astaxanthin prevents ischemia-reperfusion injury of the steatotic liver in mice.
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DOI：
10.1371/journal.pone.0187810
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Takahara S
中科院分区：
文献类型：
--
作者：
Li S;Takahara T;Fujino M;Fukuhara Y;Sugiyama T;Li XK;Takahara S
脂肪变性对缺血再灌注损伤（IRI）的耐受性较低。为了预防脂肪变性肝中的IRI，我们试图通过给小鼠喂食蛋氨酸和胆碱缺乏的高脂肪（MCDHF）饮食来阐明虾青素（ASTX）在脂肪变性肝模型中的保护作用。评估肝脏脂质过氧化和细胞凋亡水平、炎症细胞因子和血红素加氧酶(HO)-1的表达。在分离的Kupffer细胞和/或脂肪肝肝细胞中也检测了活性氧（ROS）、炎症细胞因子、凋亡相关蛋白和信号通路成员。ASTX通过IRI降低MCDHF小鼠血清ALT和AST水平，TUNEL、F4/80或4hne阳性细胞数量和炎症因子mRNA水平。此外，ASTX增加了HO-1和HIF-1α，磷酸化Akt和mTOR的表达。经缺氧和再氧化（HR）处理后，Kupffer产生的炎症细胞因子被ASTX抑制。HR增加肝细胞Bcl-2、HO-1和Nrf2的表达，而ASTX抑制Caspases的激活、Bax和ERK、MAPK、JNK的磷酸化。ASTX预处理对IRI具有保护作用，是一种安全的治疗方法，包括脂肪变性肝移植。
Steatosis has a low tolerance against ischemia-reperfusion injury (IRI). To prevent IRI in the steatotic liver, we attempted to elucidate the protective effect of astaxanthin (ASTX) in the steatotic liver model by giving mice a methionine and choline-deficient high fat (MCDHF) diet. Levels of lipid peroxidation and apoptosis, the expression of inflammatory cytokines and heme oxygenase (HO)-1, in the liver were assessed. Reactive oxygen species (ROS), inflammatory cytokines, apoptosis-related proteins and members of the signaling pathway were also examined in isolated Kupffer cells and/or hepatocytes from the steatotic liver. ASTX decreased serum ALT and AST levels, the amount of TUNEL, F4/80, or 4HNE-positive cells and the mRNA levels of inflammatory cytokines in MCDHF mice by IRI. Moreover, HO-1 and HIF-1α, phosphorylation of Akt and mTOR expressions were increased by ASTX. The inflammatory cytokines produced by Kupffer, which were subjected to hypoxia and reoxygenation (HR), were inhibited by ASTX. Expressions of Bcl-2, HO-1 and Nrf2 in hepatocytes by HR were increased, whereas Caspases activation, Bax and phosphorylation of ERK, MAPK, and JNK were suppressed by ASTX. Pretreatment with ASTX has a protective effect and is a safe therapeutic treatment for IRI, including for liver transplantation of the steatotic liver.
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DOI：
10.1007/s00534-009-0155-x
发表时间：
2009-11-01
期刊：
JOURNAL OF HEPATO-BILIARY-PANCREATIC SURGERY
影响因子：
--
作者：
King, LaShonda A.;Toledo, Alexander H.;Toledo-Pereyra, Luis H.
通讯作者：
Toledo-Pereyra, Luis H.
影响因子：
4.6
作者：
Ni Y;Nagashimada M;Zhuge F;Zhan L;Nagata N;Tsutsui A;Nakanuma Y;Kaneko S;Ota T
通讯作者：
Ota T
影响因子：
0.9
作者：
Cao, L.;Li, Y.;Long, D.
通讯作者：
Long, D.
影响因子：
6
作者：
Naito, Y;Uchiyama, K;Yoshikawa, T
通讯作者：
Yoshikawa, T
影响因子：
8.3
作者：
Lin, Wan-Ying;Chang, Ying-Chao;Huang, Chao-Ching
通讯作者：
Huang, Chao-Ching