Epigallocatechin-3O-gallate Inhibits Fibroblast Contraction of Floating Collagen Gel : Interaction between Epigallocatechin-3O-gallate and Platelet Derived Growth Factor
Epigallocatechin-3O-gallate Inhibits Fibroblast Contraction of Floating Collagen Gel : Interaction between Epigallocatechin-3O-gallate and Platelet Derived Growth Factor
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表没食子儿茶素-3O-没食子酸酯抑制漂浮胶原凝胶的成纤维细胞收缩：表没食子儿茶素-3O-没食子酸酯与血小板衍生生长因子之间的相互作用
DOI：
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
M. Isemura
中科院分区：
文献类型：
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作者：
Yasuo Suzuki;S. Hattori;M. Isemura
研究了（）-表没食子儿茶素没食子酸酯（EGCG）对成纤维细胞收缩漂浮胶原凝胶的影响。EGCG抑制胶原凝胶收缩呈剂量依赖性。基于血小板衍生生长因子（PDGF）是一种具有刺激胶原凝胶收缩活性的血清成分，我们研究了EGCG和PDGF相互作用可能参与这种抑制机制。我们在本系统中通过重组PDGF-BB证实了这一点，我们发现EGCG抑制pdgf刺激的胶原凝胶收缩。亲和层析结果表明，酶联免疫法和Western blotting检测到PDGF与固定在琼脂糖凝胶上的EGCG结合。这些发现表明，EGCG与PDGF的结合至少部分参与了EGCG抑制胶原凝胶收缩的机制。
The effects of ( )-epigallocatechin gallate (EGCG) on the contraction of floating collagen gel by fibroblasts were investigated. EGCG inhibited collagen gel contraction dose-dependently. On the basis of the fact that platelet-derived growth factor (PDGF) is one of the serum components with stimulatory activity in collagen gel contraction, we examined the possibility that interaction between EGCG and PDGF may be involved in this inhibition mechanism. We confirmed this by recombinant PDGF-BB in the present system and we found that EGCG inhibited PDGF-stimulated collagen gel contraction. The results of affinity chromatography indicated that PDGF was bound by EGCG immobilized on agarose gel as detected by enzyme-linked immunoassay and Western blotting. These findings suggest that binding of EGCG to PDGF is at least partly involved in the mechanism of inhibition of collagen gel contraction by EGCG.
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DOI：
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发表时间：
1998-04
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Chung S. Yang;Laishun Chen;Mao-jung Lee;D. Balentine;May-Chen Kuo;S. Schantz
通讯作者：
Chung S. Yang;Laishun Chen;Mao-jung Lee;D. Balentine;May-Chen Kuo;S. Schantz
体外大鼠肺成纤维细胞自发产生 PDGF A 链同二聚体。
DOI：
10.1152/ajplung.1992.263.2.l185
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
Fabisiak,JP;Absher,M;Evans,JN;Kelley,J
通讯作者：
Kelley,J
凝血酶通过其蛋白水解激活受体刺激成纤维细胞介导的胶原蛋白晶格收缩。
影响因子：
3.7
作者：
Pilcher,BK;Kim,DW;Carney,DH;Tomasek,JJ
通讯作者：
Tomasek,JJ
内源性和外源性TGF-β对大鼠肺成纤维细胞和主动脉平滑肌细胞弹性蛋白的影响。
DOI：
10.1152/ajplung.1992.263.2.l257
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
McGowan,SE
通讯作者：
McGowan,SE