Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine.
Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine.
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DOI：
10.3390/microorganisms9061315
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发表时间：
2021-06-16
期刊：
影响因子：
4.5
通讯作者：
On Behalf Of The Inmi Covid-Vaccine Study Group
中科院分区：
文献类型：
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作者：
Agrati C;Castilletti C;Goletti D;Meschi S;Sacchi A;Matusali G;Bordoni V;Petrone L;Lapa D;Notari S;Vanini V;Colavita F;Aiello A;Agresta A;Farroni C;Grassi G;Leone S;Vaia F;Capobianchi MR;Ippolito G;Puro V;On Behalf Of The Inmi Covid-Vaccine Study Group
疫苗接种是减少SARS-CoV-2传播和住院的主要公共卫生措施，全球范围内的大规模科学努力导致了有效疫苗的快速开发。这项工作的目的是确定体液和细胞介导的免疫应答的动态队列的卫生保健工作者（HCW）谁收到了两剂BNT 162 b2-mRNA疫苗接种。通过定量抗RBD和中和抗体评价血清学应答。通过全血试验定量Th 1细胞因子（IFN-γ、TNF-α、IL-2）进行细胞介导的应答，这些细胞因子是对加标肽产生的应答。BNT 162 b2-mRNA疫苗诱导体液和细胞介导的免疫反应，对刺突肽在几乎所有的HCW没有以前的SARS-CoV-2感染，在抗RBD反应与年龄呈中度负相关。刺突特异性T细胞产生几种Th 1细胞因子（IFN-γ、TNF-α和IL-2），其与特异性血清学应答相关。总之，我们的研究描述了BNT 162 b2 mRNA疫苗在HCW中引起协调的中和体液和刺突特异性T细胞应答的能力。通过简单的免疫监测方案评估这些参数的动态可以允许评估疫苗应答的持续性，以确定最佳疫苗接种策略。
Vaccination is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, and a massive worldwide scientific effort resulted in the rapid development of effective vaccines. This work aimed to define the dynamics of humoral and cell-mediated immune response in a cohort of health care workers (HCWs) who received a two-dose BNT162b2-mRNA vaccination. The serological response was evaluated by quantifying the anti-RBD and neutralizing antibodies. The cell-mediated response was performed by a whole blood test quantifying Th1 cytokines (IFN-γ, TNF-α, IL-2), produced in response to spike peptides. The BNT162b2-mRNA vaccine induced both humoral and cell-mediated immune responses against spike peptides in virtually all HCWs without previous SARS-CoV-2 infection, with a moderate inverse relation with age in the anti-RBD response. Spike-specific T cells produced several Th1 cytokines (IFN-γ, TNF-α, and IL-2), which correlated with the specific-serological response. Overall, our study describes the ability of the BNT162b2 mRNA vaccine to elicit a coordinated neutralizing humoral and spike-specific T cell response in HCWs. Assessing the dynamics of these parameters by an easy immune monitoring protocol can allow for the evaluation of the persistence of the vaccine response in order to define the optimal vaccination strategy.
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BNT162B2 mRNA COVID-19疫苗的安全性和功效。
DOI：
10.1056/nejmoa2034577
发表时间：
2020-12-31
期刊：
The New England journal of medicine
影响因子：
--
作者：
Polack FP;Thomas SJ;Kitchin N;Absalon J;Gurtman A;Lockhart S;Perez JL;Pérez Marc G;Moreira ED;Zerbini C;Bailey R;Swanson KA;Roychoudhury S;Koury K;Li P;Kalina WV;Cooper D;Frenck RW Jr;Hammitt LL;Türeci Ö;Nell H;Schaefer A;Ünal S;Tresnan DB;Mather S;Dormitzer PR;Şahin U;Jansen KU;Gruber WC;C4591001 Clinical Trial Group
通讯作者：
C4591001 Clinical Trial Group
DOI：
10.1016/j.cmi.2020.09.051
发表时间：
2021-03
期刊：
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
影响因子：
--
作者：
Petrone L;Petruccioli E;Vanini V;Cuzzi G;Najafi Fard S;Alonzi T;Castilletti C;Palmieri F;Gualano G;Vittozzi P;Nicastri E;Lepore L;Antinori A;Vergori A;Caccamo N;Cantini F;Girardi E;Ippolito G;Grifoni A;Goletti D
通讯作者：
Goletti D
影响因子：
64.5
作者：
Moderbacher, Carolyn Rydyznski;Ramirez, Sydney, I;Crotty, Shane
通讯作者：
Crotty, Shane
影响因子：
11.8
作者：
Schwarzkopf S;Krawczyk A;Knop D;Klump H;Heinold A;Heinemann FM;Thümmler L;Temme C;Breyer M;Witzke O;Dittmer U;Lenz V;Horn PA;Lindemann M
通讯作者：
Lindemann M
影响因子：
8.8
作者：
Meschi, Silvia;Colavita, Francesca;Castilletti, Concetta
通讯作者：
Castilletti, Concetta