A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients.
A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients.
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全血检测用于测量COVID-19患者的SARS-CoV-2特异性反应。
DOI：
10.1016/j.cmi.2020.09.051
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Goletti D
中科院分区：
文献类型：
--
作者：
Petrone L;Petruccioli E;Vanini V;Cuzzi G;Najafi Fard S;Alonzi T;Castilletti C;Palmieri F;Gualano G;Vittozzi P;Nicastri E;Lepore L;Antinori A;Vergori A;Caccamo N;Cantini F;Girardi E;Ippolito G;Grifoni A;Goletti D
研究是否可以使用全血实验环境在COVID-19中检测到对SARS-CoV-2肽的特异性T细胞应答，这可能进一步探索作为潜在的诊断工具。我们评估了用来自SARS-CoV-2序列的刺突抗原和抗原肽巨库（MP）刺激全血后的干扰素（IFN）-γ水平;白细胞介素（IL）-1β、IL-1 RA、IL-2、IL-4、IL-5、IL-6、IL-7、IL-8、IL-9、IL-10、IL-12 p70、IL-13、IL-15、IL-17 A、嗜酸性粒细胞趋化因子、碱性成纤维细胞生长因子（FGF），粒细胞集落刺激因子（G-CSF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、IFN-γ、干扰素γ诱导蛋白10（IP-10）、单核细胞趋化蛋白-1（MCP-1）、巨噬细胞炎性蛋白（MIP）-1α、MIP-1β、血小板衍生生长因子（PDGF）、RANTES（调节活化、正常T细胞表达和分泌）、肿瘤坏死因子-α（TNF-α）、血管内皮生长因子（VEGF）也进行了评价。与29名“非COVID-19”个体相比，35名COVID-19患者对棘突和增殖抗原MP的IFN-γ应答显著增加（中位棘突-MP：0.26 vs 0，p = 0.0002;中位增殖抗原-MP：0.07 vs 0.02; p = 0.02）。这种反应的检测与患者的临床参数无关。与“非COVID-19”个体相比，COVID-19个体对SARS-CoV-2无关抗原巨细胞病毒（CMV）和葡萄球菌肠毒素B（SE B）的IFN-γ反应相似（CMV中位数：3.46 vs 5.28，p = 0.16; SE B中位数：12.68 vs 15.05; p = 0.1）。在对COVID-19中的峰值MP的反应中-与“无COVID-19”的个体相比，我们发现IL-2的中位数显著更高，（50.08对0，p = 0.0018）、IFN-γ（90.16对0，p = 0.01）、IL-4（0.52对0，p = 0.03）、IL-13（0.84对0，p = 0.007）和MCP-1（4602对359.2，p = 0.05）。在全血测定中对SARS-CoV-2肽的免疫应答与COVID-19相关，其特征在于Th 1和Th 2谱。这种实验方法可能有助于开发新的基于T细胞的疾病和疫苗设置的诊断测试。
To examine whether specific T-cell-responses to SARS-CoV-2 peptides can be detected in COVID-19 using a whole-blood experimental setting, which may be further explored as a potential diagnostic tool. We evaluated interferon (IFN)-γ levels after stimulating whole-blood with spike and remainder-antigens peptides megapools (MP) derived from SARS-CoV-2 sequences; interleukin (IL)-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, eotaxin, basic fibroblast growth factor (FGF), granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-γ, Interferon gamma-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α, MIP-1β, Platelet-derived growth factor (PDGF), RANTES (regulated on activation, normal T cell expressed and secreted), tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF) were also evaluated. IFN-γ-response to spike and remainder-antigens MPs was significantly increased in 35 COVID-19 patients compared with 29 ‘no COVID-19’ individuals (medians spike-MP: 0.26 vs 0, p = 0.0002; medians remainder-antigens-MP: 0.07 vs 0.02; p = 0.02). This response was detected independently of patients' clinical parameters. IFN-γ-response to SARS-CoV-2-unrelated antigens cytomegalovirus (CMV) and Staphylococcal Enterotoxin B (SEB) was similar in COVID-19 compared with ‘no COVID-19’ individuals (median CMV: 3.46 vs 5.28, p = 0.16; median SEB: 12.68 vs 15.05; p = 0.1). In response to spike-MPs in COVID-19- compared with ‘no COVID-19’ -individuals, we found significant higher median of IL-2 (50.08 vs 0, p = 0.0018), IFN-γ (90.16 vs 0, p = 0.01), IL-4 (0.52 vs 0, p = 0.03), IL-13 (0.84 vs 0, p = 0.007) and MCP-1 (4602 vs 359.2, p = 0.05). Immune response to SARS-CoV-2 peptides in a whole-blood assay is associated with COVID-19 and it is characterized by both Th1 and Th2 profile. This experimental approach may be useful for developing new T-cell based diagnostic tests for disease and vaccine settings.
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影响因子：
11.8
作者：
Bordoni, Veronica;Sacchi, Alessandra;Agrati, Chiara
通讯作者：
Agrati, Chiara
HBHA特异性反应在结核病感染或不感染HIV的患者中的免疫表征。
影响因子：
3.7
作者：
Chiacchio T;Delogu G;Vanini V;Cuzzi G;De Maio F;Pinnetti C;Sampaolesi A;Antinori A;Goletti D
通讯作者：
Goletti D
影响因子：
4.1
作者：
Carrasco Pro, Sebastian;Sidney, John;Sette, Alessandro
通讯作者：
Sette, Alessandro
影响因子：
3.2
作者：
Nicastri, Emanuele;Petrosillo, Nicola;Antinori, Andrea
通讯作者：
Antinori, Andrea
DOI：
10.1128/cdli.12.11.1311-1316.2005
发表时间：
2005-11-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Goletti, D;Vincenti, D;Girardi, E
通讯作者：
Girardi, E