Direct detection of adenosine in undiluted serum using a luminescent aptamer sensor attached to a terbium complex.
Direct detection of adenosine in undiluted serum using a luminescent aptamer sensor attached to a terbium complex.
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DOI：
10.1021/ac302167d
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发表时间：
2012-09-18
影响因子：
7.4
通讯作者：
Lu, Yi
中科院分区：
文献类型：
--
作者：
Li, Le-Le;Ge, Pinghua;Selvin, Paul R.;Lu, Yi
核酸适体是一种能选择性地与多种靶分子结合的单链核酸，已被广泛用于构建生物传感器。然而，该领域的一个主要挑战是直接感测复杂生物介质中的分析物，例如未稀释的血清。虽然在通过使用预分离步骤来洗去血清中的干扰物而开发非均质测定法方面已经取得了进展，但是高度期望用于直接检测均质未加工血清中的靶标的简易策略。我们在此报告了一种可直接检测未稀释和未处理的血清中腺苷的开启发光适体生物传感器，通过利用具有长发光寿命的铽螯合物来实现时间分辨检测。该传感器的检测限为60 µM腺苷，同时具有与缓冲液相当的卓越选择性。这里展示的方法可以应用于直接检测和定量的生物介质中的广泛的分析物，通过使用其他适配体。
Aptamers, single-stranded nucleic acids that can selectively bind to various target molecules, have been widely used for constructing biosensors. A major challenge in this field, however, is direct sensing of analytes in complex biological media such as undiluted serum. While progress has been made in developing inhomogeneous assay by using a pre-separation step to wash away the interferences within serum, a facile strategy for direct detection of targets in homogenous unprocessed serum is highly desired. We herein report a turn-on luminescent aptamer biosensor for the direct detection of adenosine in undiluted and unprocessed serum, by taking advantage of a terbium chelate complex with long luminescence lifetime to achieve time-resolved detection. The sensor exhibits a detection limit of 60 µM adenosine while marinating excellent selectivity that is comparable to those in buffer. The approach demonstrated here can be applied for direct detection and quantification of a broad range of analytes in biological media by using other aptamers.
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影响因子：
62.1
作者：
Liu, Juewen;Cao, Zehui;Lu, Yi
通讯作者：
Lu, Yi
用于生物体液中核酸高灵敏检测的室温磷光分子信标的设计
影响因子：
7.4
作者：
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通讯作者：
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影响因子：
46.9
作者：
Jhaveri, S;Rajendran, M;Ellington, AD
通讯作者：
Ellington, AD
影响因子：
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作者：
Liu, Juewen;Lee, Jung Heon;Lu, Yi
通讯作者：
Lu, Yi
影响因子：
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作者：
Chen, JY;Selvin, PR
通讯作者：
Selvin, PR