Responses to ethanol in C57BL/6 versus C57BL/6 × 129 hybrid mice.
Responses to ethanol in C57BL/6 versus C57BL/6 × 129 hybrid mice.
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DOI：
10.1002/brb3.29
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发表时间：
2012-01
影响因子：
3.1
通讯作者：
Messing, Robert O.
中科院分区：
文献类型：
--
作者：
Lim, Jana P.;Zou, Mimi E.;Janak, Patricia H.;Messing, Robert O.
虽然遗传背景改变了对乙醇的反应，但基因靶向研究中常用的近交系和杂交小鼠之间乙醇相关行为的差异尚未得到系统的量化。在此，我们在五项常用试验中比较了C57 BL/6 NTac × 129 S6/SvEvTac F1杂交小鼠（B6129 S6）与C57 BL/6 NTac近交系小鼠（B6 NT），以及C57 BL/6 J × 129 X1/SvJ（B6129 X1）和C57 BL/6 J × 129 S4/SvJae F1杂交小鼠（B6129 S4）与C57 BL/6 J小鼠（B6 J）：连续性两瓶选择饮酒、间歇性限制性狂饮、乙醇清除、乙醇诱导的翻正反射丧失和乙醇条件性位置偏好（CPP）。我们发现，近交系B6 J和B6 NT小鼠表现出更大的乙醇偏好和消费比各自的杂交种时，乙醇是连续可用的。在间歇性限制饮酒过程中，尽管所有品系在8次饮酒过程中的摄入量相似，但所有饮酒过程的平均值显示，B6 NT小鼠比B6129 S6小鼠喝了更多的乙醇。此外，B6 J小鼠比B6129 X1小鼠消耗更多的乙醇，尽管它们比B6129 S4小鼠喝得少。在近交系和杂交小鼠之间没有观察到乙醇LORR持续时间的差异。虽然B6 J小鼠和它们各自的杂交种之间的乙醇清除率相似，但B6 NT小鼠比B6129 S6小鼠更快地清除乙醇。所有生产线都开发了乙醇CPP。我们的研究结果表明，它可能是没有必要的回交杂交到近交B6背景研究基因靶向小鼠的许多乙醇相关的行为。
Although genetic background alters responses to ethanol, there has not yet been a methodical quantification of differences in ethanol-related behaviors between inbred and hybrid mice commonly used in gene-targeting studies. Here, we compared C57BL/6NTac × 129S6/SvEvTac F1 hybrid mice (B6129S6) with C57BL/6NTac inbred mice (B6NT), and C57BL/6J × 129X1/SvJ (B6129X1) and C57BL/6J × 129S4/SvJae F1 hybrids (B6129S4) with C57BL/6J mice (B6J), in five commonly used tests: continuous access two-bottle choice drinking, intermittent limited-access binge drinking, ethanol clearance, ethanol-induced loss of the righting reflex, and conditioned place preference (CPP) for ethanol. We found that inbred B6J and B6NT mice showed greater ethanol preference and consumption than their respective hybrids when ethanol was continuously available. Within the intermittent limited-access drinking procedure, though all lines showed similar intake over eight drinking sessions, the average of all sessions showed that B6NT mice drank significantly more ethanol than B6129S6 mice. In addition, B6J mice consumed more ethanol than B6129X1 mice, although they drank less than B6129S4 mice. No differences in ethanol LORR duration were observed between inbred and hybrid mice. Although ethanol clearance was similar among B6J mice and their respective hybrids, B6NT mice cleared ethanol more rapidly than B6129S6 mice. All lines developed CPP for ethanol. Our findings indicate that it may not be necessary to backcross hybrids to an inbred B6 background to study many ethanol-related behaviors in gene-targeted mice.
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影响因子：
2.5
作者：
Bothe, GWM;Bolivar, VJ;Geistfeld, JG
通讯作者：
Geistfeld, JG
DOI：
10.1073/pnas.1005554107
发表时间：
2010-11-16
影响因子：
11.1
作者：
Neasta, Jeremie;Hamida, Sami Ben;Ron, Dorit
通讯作者：
Ron, Dorit
DOI：
10.1097/00000374-199904001-00015
发表时间：
1999-04-01
影响因子：
3.2
作者：
Nocjar, C;Middaugh, LD;Tavernetti, M
通讯作者：
Tavernetti, M
影响因子：
30.8
作者：
Simpson, EM;Linder, CC;Sharp, JJ
通讯作者：
Sharp, JJ
影响因子：
2.9
作者：
Rhodes, JS;Best, K;Crabbe, JC
通讯作者：
Crabbe, JC