Fetal bovine serum xenoproteins modulate human monocyte adhesion and protein release on biomaterials in vitro.
Fetal bovine serum xenoproteins modulate human monocyte adhesion and protein release on biomaterials in vitro.
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胎牛血清异种蛋白在体外调节生物材料上的人单核细胞粘附和蛋白质释放。
DOI：
10.1016/j.actbio.2010.08.022
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发表时间：
2011
影响因子：
9.7
通讯作者：
Kao,WeiyuanJohn
中科院分区：
文献类型：
--
作者：
Schmidt,David;Joyce,EvanJames;Kao,WeiyuanJohn
Monocyte-derived macrophages are critical in the host–foreign body response to biomaterials and have been studied extensively in various culture conditions in vitro, such as medium supplemented with fetal bovine serum (FBS) or autologous human serum (AHS). Since monocyte maturation into macrophages is highly plastic and may vary considerably depending on the surface, isolation procedures and in vitro culture conditions, we hypothesize that variations in protein adsorption and serum type will greatly impact monocyte behavior in a surface-dependent manner. The impact of xenoproteins on monocyte-surface interactions has not been well studied methodically and the use of AHS rather than FBS for macrophage-biomaterials studies in vitro is far from universal. The commonly used reference materials – tissue culture polystyrene (TCPS), polyethylene glycol (PEG) and polydimethylsiloxane (PDMS) – were employed in this study and we found a 3-fold higher adherent monocyte density on TCPS when AHS was used vs. FBS-supplemented medium. On PEG hydrogels, an 8- to 10-fold higher adhesion density was observed when AHS was employed vs. FBS, while on PDMS no difference in adhesion density was observed between the two sera conditions. Additionally, the presence of lipopolysaccharide abrogated the serum-dependent effect on cell adhesion on TCPS. Significantly different variations in protein release were observed between the serum conditions on these surfaces; in particular, there was a 100-fold higher concentration of growth-related oncogene for the AHS condition on PDMS even though the adhesion levels were comparable between the two serum conditions. These results emphasize the combined impact of the surface type and FBS xenoproteins in mediating the observed monocyte response to biomaterials in vitro.
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影响因子：
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DOI：
--
发表时间：
1971
期刊：
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影响因子：
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作者：
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DOI：
10.1152/ajpcell.1983.245.5.c388
发表时间：
1983
期刊：
The American journal of physiology
影响因子：
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作者：
Widdicombe,JH;Nathanson,IT;Highland,E
通讯作者：
Highland,E
影响因子：
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作者：
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DOI：
10.1016/0005-2736(83)90056-1
发表时间：
1983
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
C. Festen;J. Slegers;C. van Os
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