Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus.
Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus.
复制标题
精液细胞外囊泡介导亚群禽白血病病毒的垂直传播
DOI：
10.1016/j.virs.2022.01.026
复制
发表时间：
2022-04
影响因子：
5.5
通讯作者：
Xie, Qingmei
中科院分区：
文献类型：
--
作者：
Liao, Liqin;Chen, Weiguo;Zhang, Xiangyu;Zhang, Huanmin;Li, Aijun;Yan, Yiming;Xie, Zi;Li, Hongxing;Lin, Wencheng;Ma, Jingyun;Zhang, Xinheng;Xie, Qingmei
关键词：
J亚群禽白血病病毒（ALV - J）是一种高度致癌的逆转录病毒，自20世纪90年代末以来一直在全球家禽业造成严重破坏。ALV - J的主要感染模式是垂直传播，这导致了子代代代先天性感染。越来越多的证据表明，来自病毒感染细胞或生物体液的细胞外囊泡（EVs）被认为是病毒传播的载体。然而，EVs在ALV - J感染和传播中的作用仍不清楚。在本研究中，从感染ALV - J的公鸡精浆中分离和纯化了精液细胞外囊泡（SE）（SE - ALV - J），通过RNA测序、逆转录定量PCR和蛋白质印迹分析确定其含有ALV - J基因组RNA和部分病毒蛋白。此外，SE - ALV - J被证明能够将ALV - J感染传播给宿主细胞并建立生产性感染。更重要的是，人工授精实验表明，SE - ALV - J将ALV - J感染传播给无特定病原体（SPF）母鸡，并随后介导了ALV - J从SPF母鸡到子代雏鸡的垂直传播。综上所述，本研究结果表明ALV - J利用宿主精液细胞外囊泡作为一种新的垂直传播方式，增进了我们对ALV - J传播机制的理解。
SE - ALV - J含有ALV - J基因组RNA和部分病毒蛋白。
SE - ALV - J能够将ALV - J感染传播给宿主细胞并建立生产性感染。
SE - ALV - J在动物模型中介导ALV - J的垂直传播。
Subgroup J avian leukosis virus (ALV-J) is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s. The major infection model of ALV-J is vertical transmission, which is responsible for the congenital infection of progeny from generation to generation. Increasing evidence has suggested that extracellular vesicles (EVs) derived from virus-infected cells or biological fluids have been thought to be vehicles of transmission for viruses. However, the role of EVs in infection and transmission of ALV-J remains obscure. In the present study, semen extracellular vesicles (SE) were isolated and purified from ALV-J-infected rooster seminal plasma (SE-ALV-J), which was shown to contain ALV-J genomic RNA and partial viral proteins, as determined by RNA sequencing, reverse transcription-quantitative PCR and Western blotting. Furthermore, SE-ALV-J was proved to be able to transmit ALV-J infection to host cells and establish productive infection. More importantly, artificial insemination experiments showed that SE-ALV-J transmitted ALV-J infection to SPF hens, and subsequently mediated vertical transmission of ALV-J from the SPF hens to the progeny chicks. Taken together, the results of the present study suggested that ALV-J utilized host semen extracellular vesicles as a novel means for vertical transmission, enhancing our understanding on mechanisms underlying ALV-J transmission. SE-ALV-J- contains ALV-J genomic RNA and partial viral proteins. SE-ALV-J could transmit ALV-J infection to host cells and establish productive infection. SE-ALV-J mediates vertical transmission of ALV-J in animal model.
登录
查看更多内容
DOI：
10.3390/v7062770
发表时间：
2015-06-19
期刊：
Viruses
影响因子：
--
作者：
Chahar HS;Bao X;Casola A
通讯作者：
Casola A
DOI：
10.1126/science.aau6977
发表时间：
2020-02-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kalluri R;LeBleu VS
通讯作者：
LeBleu VS
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
1.7
作者：
Kechin, Andrey;Boyarskikh, Uljana;Filipenko, Maxim
通讯作者：
Filipenko, Maxim
影响因子：
4.8
作者：
Lin Y;Xia J;Zhao Y;Wang F;Yu S;Zou N;Wen X;Cao S;Huang Y
通讯作者：
Huang Y