The MADS-Box transcription factor Bcmads1 is required for growth, sclerotia production and pathogenicity of Botrytis cinerea.
The MADS-Box transcription factor Bcmads1 is required for growth, sclerotia production and pathogenicity of Botrytis cinerea.
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MADS-Box 转录因子 Bcmads1 是灰霉病菌生长、菌核产生和致病性所必需的
DOI：
10.1038/srep33901
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发表时间：
2016-09-23
影响因子：
4.6
通讯作者：
Tian S
中科院分区：
文献类型：
--
作者：
Zhang Z;Li H;Qin G;He C;Li B;Tian S
MADS盒转录因子在真核生物中高度保守，参与多种生物学过程。然而，关于灰葡萄孢（Botrytis cinerea）中MADS盒基因的功能知之甚少，灰葡萄孢是一种具有广泛宿主范围的真菌病原体。这里，B的功能作用。cinerea MADS-box基因Bcmads 1与菌核的发育、致病性和产生有关。后者在黑暗中孵化时形成，在冬季作为生存结构，并在有性生殖中作为雌性亲本。bcmads 1是菌核产生所必需的。RT-qPCR分析表明Bcmads 1通过调控光响应基因的表达来调控菌核的形成。Bcmads 1是B的全部毒力潜力所必需的。苹果果实上的灰霉病比较蛋白质组学分析鉴定了63种蛋白质，代表了Bcmads 1潜在靶点的55个基因。其中，Bcsec 14和Bcsec 31与囊泡运输相关。Bcsec 14和Bcsec 31的缺失导致B的毒力和蛋白分泌降低。灰叶这些结果表明，Bcmads 1可能通过调节光响应基因的表达来影响菌核的形成，并通过其对蛋白质分泌过程的影响来调节致病性。
MADS-box transcription factors are highly conserved in eukaryotic species and involved in a variety of biological processes. Little is known, however, regarding the function of MADS-box genes in Botrytis cinerea, a fungal pathogen with a wide host range. Here, the functional role of the B. cinerea MADS-box gene, Bcmads1, was characterized in relation to the development, pathogenicity and production of sclerotia. The latter are formed upon incubation in darkness and serve as survival structures during winter and as the female parent in sexual reproduction. Bcmads1 is indispensable for sclerotia production. RT-qPCR analysis suggested that Bcmads1 modulated sclerotia formation by regulating the expression of light-responsive genes. Bcmads1 is required for the full virulence potential of B. cinerea on apple fruit. A comparative proteomic analysis identified 63 proteins, representing 55 individual genes that are potential targets of Bcmads1. Among them, Bcsec14 and Bcsec31 are associated with vesicle transport. Deletion of Bcsec14 and Bcsec31 resulted in a reduction in the virulence and protein secretion of B. cinerea. These results suggest that Bcmads1 may influence sclerotia formation by modulating light responsive gene expression and regulate pathogenicity by its effect on the protein secretion process.
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影响因子：
11.3
作者：
Baker CL;Loros JJ;Dunlap JC
通讯作者：
Dunlap JC
影响因子：
--
作者：
Mehrabi, Rahim;Ding, Shengli;Xu, Jin-Rong
通讯作者：
Xu, Jin-Rong
DOI：
10.1083/jcb.108.4.1271
发表时间：
1989-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bankaitis VA;Malehorn DE;Emr SD;Greene R
通讯作者：
Greene R
影响因子：
3
作者：
Li, Moyi;Rollins, Jeffrey A.
通讯作者：
Rollins, Jeffrey A.
影响因子：
4.8
作者：
Curwin, Amy J.;Fairn, Gregory D.;McMaster, Christopher R.
通讯作者：
McMaster, Christopher R.