Delineating species with DNA barcodes: a case of taxon dependent method performance in moths.
Delineating species with DNA barcodes: a case of taxon dependent method performance in moths.
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DOI：
10.1371/journal.pone.0122481
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hebert PD
中科院分区：
文献类型：
--
作者：
Kekkonen M;Mutanen M;Kaila L;Nieminen M;Hebert PD
生物多样性的加速丧失需要更有效的方法来发现物种。用于分析序列数据的新算法方法与快速扩展的DNA条形码文库相结合提供了潜在的解决方案。虽然有几种分析方法可用于划分操作分类单位，但很少有研究比较它们的性能。本研究比较了一种基于形态学的方法和四种基于DNA的方法（BIN、简约网络、ABGD、GMYC）对两组麦蛾的识别效果。本文对传统分类学所划分出的92种芬兰长刺叶蜂亚科和103种澳大利亚长刺叶蜂亚科进行了研究。结果显示，这两个类群与所有四个基于DNA的方法之间的性能显着差异。在Elachistinae的OTU计数显示了较宽的范围和相对较低的（约。65%）OTU与参考物种匹配，而OTU计数更一致，性能更高（约65%）。90%）。性能上升时，只有单系物种进行比较，但分类依赖性仍然存在。基于DNA的方法都不能与非单系物种产生正确的匹配，但单系物种处理得很好。形态种分组的模拟测试表现得很差，揭示这些小的，沉闷的颜色蛾类群的多样性。尽管基于DNA条形码的分析性能强劲，但使用单基因座线粒体DNA数据描绘的物种最好被视为需要后续综合分类学工作验证的OTU。
The accelerating loss of biodiversity has created a need for more effective ways to discover species. Novel algorithmic approaches for analyzing sequence data combined with rapidly expanding DNA barcode libraries provide a potential solution. While several analytical methods are available for the delineation of operational taxonomic units (OTUs), few studies have compared their performance. This study compares the performance of one morphology-based and four DNA-based (BIN, parsimony networks, ABGD, GMYC) methods on two groups of gelechioid moths. It examines 92 species of Finnish Gelechiinae and 103 species of Australian Elachistinae which were delineated by traditional taxonomy. The results reveal a striking difference in performance between the two taxa with all four DNA-based methods. OTU counts in the Elachistinae showed a wider range and a relatively low (ca. 65%) OTU match with reference species while OTU counts were more congruent and performance was higher (ca. 90%) in the Gelechiinae. Performance rose when only monophyletic species were compared, but the taxon-dependence remained. None of the DNA-based methods produced a correct match with non-monophyletic species, but singletons were handled well. A simulated test of morphospecies-grouping performed very poorly in revealing taxon diversity in these small, dull-colored moths. Despite the strong performance of analyses based on DNA barcodes, species delineated using single-locus mtDNA data are best viewed as OTUs that require validation by subsequent integrative taxonomic work.
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