Recovery of the Gut Microbiota after Antibiotics Depends on Host Diet, Community Context, and Environmental Reservoirs.
Recovery of the Gut Microbiota after Antibiotics Depends on Host Diet, Community Context, and Environmental Reservoirs.
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DOI：
10.1016/j.chom.2019.10.011
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发表时间：
2019-11-13
影响因子：
30.3
通讯作者：
Huang KC
中科院分区：
文献类型：
--
作者：
Ng KM;Aranda-Díaz A;Tropini C;Frankel MR;Van Treuren W;O'Loughlin CT;Merrill BD;Yu FB;Pruss KM;Oliveira RA;Higginbottom SK;Neff NF;Fischbach MA;Xavier KB;Sonnenburg JL;Huang KC
抗生素改变了微生物群的组成，增加了感染的敏感性。然而，抗生素对肠道共生的普遍影响以及环境变量的作用仍不清楚。为了解决这个问题，我们在受控的小鼠系统中跟踪了抗生素治疗期间具有高时间和分类分辨率的微生物区系动态，隔离了诸如饮食、治疗史和住房共同居民等变量。人类微生物区系具有显著的弹性，并在抗生素治疗期间恢复，耐药类杆菌暂时占优势，分类群不对称多样性减少。在某些情况下，体外敏感性不能预测体内反应，强调了宿主和社区背景的重要性。缺乏纤维的饮食加剧了微生物区系的崩溃和恢复的延迟。环丙沙星治疗后通过交叉安置的物种替换建立了对第二次治疗的复原力。单一住房严重扰乱了经济复苏，突显了环境水库的重要性。我们的发现突出了确定的微生物区系对扰动的适应，以及在抗生素期间调节饮食、卫生和微生物区系组成的翻译潜力。Ng等人的研究成果。系统地比较小鼠的微生物群对抗生素的反应。缺乏纤维的饮食或单一的住房加剧了原本迅速恢复的情况，而通过重复治疗或在宿主之间传输的微生物区系重新编程增强了复原力。这项工作突出了在抗生素治疗期间调节饮食、卫生和微生物区系组成的翻译潜力。
Antibiotics alter microbiota composition and increase infection susceptibility. However, the generalizable effects of antibiotics on gut commensals and the contribution of environmental variables remain unclear. To address this, we tracked microbiota dynamics with high temporal and taxonomic resolution during antibiotic treatment in a controlled murine system, isolating variables such as diet, treatment history, and housing co-inhabitants. Human microbiota were remarkably resilient, and recovered during antibiotic treatment with transient dominance of resistant Bacteroides and taxa-asymmetric diversity reduction. In certain cases, in vitro sensitivities were not predictive of in vivo responses, underscoring the significance of host and community context. A fiber-deficient diet exacerbated microbiota collapse and delayed recovery. Species replacement through cross-housing after ciprofloxacin treatment established resilience to a second treatment. Single-housing drastically disrupted recovery, highlighting the importance of environmental reservoirs. Our findings highlight deterministic microbiota adaptations to perturbations, and the translational potential for modulating diet, sanitation, and microbiota composition during antibiotics. Ng et al. systematically compare microbiota responses to antibiotics in mice. A fiber-deficient diet or single-housing aggravated otherwise rapid recovery, while microbiota reprogramming through repeated treatment or transmission between hosts enhanced resilience. This work highlights the translational potential for modulating diet, sanitation, and microbiota composition during antibiotic treatment.
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影响因子：
4.9
作者：
Karlowsky, James A.;Walkty, Andrew J.;Zhanel, George G.
通讯作者：
Zhanel, George G.
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1007/978-1-61779-513-8_13
发表时间：
2012-01-01
期刊：
MUCINS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Johansson, Malin E. V.;Hansson, Gunnar C.
通讯作者：
Hansson, Gunnar C.
影响因子：
64.5
作者：
Cox LM;Yamanishi S;Sohn J;Alekseyenko AV;Leung JM;Cho I;Kim SG;Li H;Gao Z;Mahana D;Zárate Rodriguez JG;Rogers AB;Robine N;Loke P;Blaser MJ
通讯作者：
Blaser MJ