DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber Green Mottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber Green Mottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
复制标题
通过全基因组亚硫酸氢盐测序分析西瓜对黄瓜绿斑驳花叶病毒感染的反应的 DNA 甲基化
DOI：
10.3390/genes10050344
复制
发表时间：
2019-05
期刊：
影响因子：
3.5
通讯作者：
Yanjun He
中科院分区：
文献类型：
--
作者：
Yeyan Sun;Min Fan;Yanjun He
DNA甲基化是与植物免疫相关的重要表观遗传标记，但其在西瓜病毒感染中的作用尚不清楚。我们对接种黄瓜绿色斑驳花叶病毒（CGMMV）后0 h（CK）、48 h和25 d的西瓜叶片进行了全基因组亚硫酸氢盐测序。在CGMMV感染过程中，差异甲基化区域（DMR）的数量增加，从3个文库中筛选出2788个DMR相关基因（DMG）。大多数DMR和DMG是在CHH背景下获得的。这些DMG在京都基因和基因组百科全书（KEGG）的次生生物合成和代谢、植物-病原体相互作用、Toll样受体信号传导和ABC转运蛋白途径中显著富集。此外，编码PR 1a、CaMs、钙结合蛋白、RIN 4、BAK 1、WRKY、RBOH、STK和RLP/RLK的DMG参与西瓜-CGMMV相互作用和信号传导。通过RNA-seq分析DNA甲基化和基因表达之间的关联，未检测到明确的关系。此外，RdDM途径中基因的下调表明减少的RdDM指导的CHH甲基化在西瓜的抗病毒防御中起重要作用。我们的研究结果提供了西瓜全基因组DNA甲基化谱，将有助于揭示在甲基化水平上响应CGMMV感染的分子机制。
DNA methylation is an important epigenetic mark associated with plant immunity, but little is known about its roles in viral infection of watermelon. We carried out whole-genome bisulfite sequencing of watermelon leaves at 0 h (ck), 48 h, and 25 days post-inoculation with Cucumber green mottle mosaic virus (CGMMV). The number of differentially methylated regions (DMRs) increased during CGMMV infection and 2788 DMR-associated genes (DMGs) were screened out among three libraries. Most DMRs and DMGs were obtained under the CHH context. These DMGs were significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of secondary biosynthesis and metabolism, plant–pathogen interactions, Toll-like receptor signaling, and ABC transporters. Additionally, DMGs encoding PR1a, CaMs, calcium-binding protein, RIN4, BAK1, WRKYs, RBOHs, STKs, and RLPs/RLKs were involved in the watermelon–CGMMV interaction and signaling. The association between DNA methylation and gene expression was analyzed by RNA-seq and no clear relationship was detected. Moreover, downregulation of genes in the RdDM pathway suggested the reduced RdDM-directed CHH methylation plays an important role in antiviral defense in watermelon. Our findings provide genome-wide DNA methylation profiles of watermelon and will aid in revealing the molecular mechanism in response to CGMMV infection at the methylation level.
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DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
W. F. Bewley
通讯作者：
W. F. Bewley
影响因子：
16.8
作者：
Stroud H;Do T;Du J;Zhong X;Feng S;Johnson L;Patel DJ;Jacobsen SE
通讯作者：
Jacobsen SE
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
DOI：
10.3390/v10080402
发表时间：
2018-07-29
期刊：
Viruses
影响因子：
--
作者：
Wang C;Wang C;Xu W;Zou J;Qiu Y;Kong J;Yang Y;Zhang B;Zhu S
通讯作者：
Zhu S
影响因子：
4.3
作者：
Seung-Yeol Lee;N. K. Win;Donghwi Cho;Su-Heon Lee;H. Jung
通讯作者：
Seung-Yeol Lee;N. K. Win;Donghwi Cho;Su-Heon Lee;H. Jung