Epigenetic Changes in the Regulation of Nicotiana tabacum Response to Cucumber Mosaic Virus Infection and Symptom Recovery through Single-Base Resolution Methylomes.
Epigenetic Changes in the Regulation of Nicotiana tabacum Response to Cucumber Mosaic Virus Infection and Symptom Recovery through Single-Base Resolution Methylomes.
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DOI：
10.3390/v10080402
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发表时间：
2018-07-29
期刊：
影响因子：
--
通讯作者：
Zhu S
中科院分区：
文献类型：
--
作者：
Wang C;Wang C;Xu W;Zou J;Qiu Y;Kong J;Yang Y;Zhang B;Zhu S
植物已经进化出多种机制来应对病毒感染。这些反应已在宿主免疫反应和抗病毒RNA沉默（RNAi）水平上详细研究。然而，表观遗传重编程的可能性尚未得到彻底研究。在这里，我们确定了病毒感染过程中DNA甲基化的作用，并进行了减少代表亚硫酸氢盐测序（RRBS）的黄瓜花叶病毒（CMV）感染的烟草在不同发育阶段的组织。差异甲基化区域富含CHH序列背景，其中80%位于基因体上以时间方式调节基因表达。甲基化基因的甲基转移酶抑制抑制剂在很大程度上重叠的甲基化基因的病毒入侵。Argonaute蛋白的激活和甲基供体合酶的抑制揭示了动态甲基化变化在调节病毒清除和抗性信号传导中的重要作用。发现甲基化-表达关系是免疫应答所必需的，并且细胞组分是对感染和症状恢复的适当防御应答所必需的。
Plants have evolved multiple mechanisms to respond to viral infection. These responses have been studied in detail at the level of host immune response and antiviral RNA silencing (RNAi). However, the possibility of epigenetic reprogramming has not been thoroughly investigated. Here, we identified the role of DNA methylation during viral infection and performed reduced representation bisulfite sequencing (RRBS) on tissues of Cucumber mosaic virus (CMV)-infected Nicotiana tabacum at various developmental stages. Differential methylated regions are enriched with CHH sequence contexts, 80% of which are located on the gene body to regulate gene expression in a temporal style. The methylated genes depressed by methyltransferase inhibition largely overlapped with methylated genes in response to viral invasion. Activation in the argonaute protein and depression in methyl donor synthase revealed the important role of dynamic methylation changes in modulating viral clearance and resistance signaling. Methylation-expression relationships were found to be required for the immune response and cellular components are necessary for the proper defense response to infection and symptom recovery.
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DOI：
10.1073/pnas.1201043109
发表时间：
2012-02-28
影响因子：
11.1
作者：
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通讯作者：
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DOI：
10.1038/nrm4043
发表时间：
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期刊：
Nature reviews. Molecular cell biology
影响因子：
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作者：
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影响因子：
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通讯作者：
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影响因子：
3.7
作者：
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DOI：
10.1073/pnas.1209329109
发表时间：
2012-08-07
影响因子：
11.1
作者：
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通讯作者：
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