Sleep dysfunction following neonatal ischemic seizures are differential by neonatal age of insult as determined by qEEG in a mouse model.
Sleep dysfunction following neonatal ischemic seizures are differential by neonatal age of insult as determined by qEEG in a mouse model.
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新生儿缺血性癫痫发作后的睡眠功能障碍是由QEEG在小鼠模型中确定的侮辱年龄的差异。
DOI：
10.1016/j.nbd.2018.04.012
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发表时间：
2018-08
影响因子：
6.1
通讯作者：
Kadam SD
中科院分区：
文献类型：
--
作者：
Kang SK;Ammanuel S;Thodupunuri S;Adler DA;Johnston MV;Kadam SD
与缺氧缺血性脑病（HIE）相关的新生儿癫痫发作对其急性临床治疗提出了挑战，并且通常会导致长期的神经系统后果。我们使用了新鉴定的 CD-1 小鼠新生儿缺血性癫痫模型，该模型与单侧颈动脉结扎后年龄依赖性（P7 与 P10）癫痫严重程度和苯巴比妥疗效（即分别为 PB 耐药与 PB 有效）相关。使用神经行为测试、24 小时垂直定量 EEG-EMG（qEEG、qEMG）和成年小鼠的蛋白质印迹分析，研究了 P7 与 P10 结扎幼仔未经治疗的新生儿癫痫发作的长期后果。两个年龄组的一小部分小鼠都出现了明显的过度活跃，这与旷场（OF）测试期间未能适应相关。 24 小时连续 qEEG 检测到，由于两个年龄组的长觉醒周期，睡眠结构发生了显着改变。慢波睡眠 (SWS) 期间的 Delta 功率 (0.5-4 Hz) 定量显示，在睡眠压力增加期间，P10 连接体中存在显着的 SWS 补偿，而 P7 连接体组未能显示。被视为注意力控制负相关标记的 Theta/β 比率仅在 P10 连接体中显着较高。这些结果表明，当用成人 v-qEEG/EMG 进行评估时，CD-1 幼犬缺血性新生儿癫痫发作特征的新生儿年龄依赖性差异对长期结果有不同的调节作用。
Neonatal seizures associated with hypoxic-ischemic encephalopathy (HIE) pose a challenge in their acute clinical management and are often followed by long-term neurological consequences. We used a newly characterized CD-1 mouse model of neonatal ischemic seizures associated with age-dependent (P7 vs. P10) seizure severity and phenobarbital efficacy (i.e.; PB-resistant vs. PB-efficacious respectively) following unilateral carotid ligation. The long-term consequences following untreated neonatal seizures in P7 vs. P10 ligated pups were investigated using neurobehavioral testing, 24h v- quantitative EEG-EMG (qEEG, qEMG), and western blot analyses in adult mice. Significant hyperactivity emerged in a small sub-set of mice in both age-groups associated with a failure to habituate during open-field (OF) testing. 24h continuous qEEGs detected significantly altered sleep architecture due to long-wake cycles in both age-groups. Delta power (0.5-4 Hz) quantification during slow-wave-sleep (SWS) revealed significant SWS compensation in P10 ligates following periods of increased sleep pressure which the P7 ligate group failed to show. Theta/beta ratios deemed as negative correlation markers of attentional control were significantly higher only in the P10 ligates. These results indicate that neonatal age-dependent differences in the characteristics of ischemic neonatal seizures in CD-1 pups differentially modulate long-term outcomes, when evaluated with v-qEEG/EMG as adults.
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影响因子：
5.3
作者：
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作者：
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