Abscisic acid enhances tolerance to spring freeze stress and regulates the expression of ascorbate–glutathione biosynthesis-related genes and stress-responsive genes in common wheat
Abscisic acid enhances tolerance to spring freeze stress and regulates the expression of ascorbate–glutathione biosynthesis-related genes and stress-responsive genes in common wheat
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脱落酸增强普通小麦对春季冻害的耐受性并调节抗坏血酸-谷胱甘肽生物合成相关基因和逆境响应基因的表达
DOI：
10.1007/s11032-020-01187-9
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发表时间：
2020-11
影响因子：
3.1
通讯作者：
Chen Feng
中科院分区：
文献类型：
--
作者：
Zhang Ning;Li Linjie;Zhang Lingran;Li Jia;Fang Yuhui;Zhao Lei;Ren Yan;Chen Feng
研究表明，外源脱落酸（阿坝）预处理显著提高了普通小麦（Triticum aestivumL.）幼苗花药结缔组织形成期和四分体发育期。阿坝预处理显著提高了低温胁迫3d后叶片中可溶性糖、可溶性蛋白质、脯氨酸含量以及超氧化物歧化酶（SOD）和过氧化物酶（POD）活性，降低了过氧化氢（H2 O2）和丙二醛（MDA）含量。在冷冻胁迫条件下，阿坝处理在ACFP和四分体发育阶段导致叶片抗坏血酸（阿萨）和谷胱甘肽（GSH）水平显着增加。采用实时定量PCR（qRT-PCR）技术检测了8个ASA-GSH合成相关酶基因和8个胁迫响应基因的时空表达模式。结果表明，阿坝对这些基因的转录水平具有时间调控作用。此外，这些基因在ABA处理的小麦幼苗之间的ACFP和四分体发育阶段在冷冻胁迫期间的差异表达。这些结果表明，外源阿坝以时间特异性和发育阶段特异性的方式增加了冷冻胁迫下小麦幼苗中GSH和阿萨的水平。此外，我们在本研究中发现，ABA非依赖性途径和ABA依赖性途径并不完全独立，并且在至少一个胁迫暴露时间点，ABA非依赖性胁迫响应基因也受到外源阿坝的诱导。我们的研究结果与以前的研究结果的比较表明，植物激素诱导GSH和阿萨水平的增加，可能是通过差异调节编码ASA-GSH合成酶的基因的表达水平。本研究结果首次揭示了ABA诱导小麦耐春冻性的分子机制。
The present study showed that exogenous abscisic acid (ABA) pre-treatment remarkably increases the freeze tolerance of common wheat (Triticum aestivumL.) seedlings at the anther connective tissue formation phase (ACFP) and tetrad developmental phase. After 3-day freeze stress, ABA pre-treatment significantly increases the content of soluble sugar, soluble protein, and proline, activities of superoxide dismutase (SOD) and peroxidase (POD) and decreases hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels. Under freeze stress conditions, ABA treatment at the ACFP and tetrad developmental phase resulted in a marked increase in leaf ascorbic acid (ASA) and glutathione (GSH) levels. Temporal and spatial expression patterns of eight genes encoding ASA–GSH synthesis-related enzymes and eight stress-responsive genes were measured using quantitative real-time PCR (qRT-PCR). The results showed that ABA temporally regulated the transcript levels of these genes. Moreover, these genes were differentially expressed in ABA-treated wheat seedlings between the ACFP and tetrad developmental phase during freeze stress. These results implied that exogenous ABA increases the levels of GSH and ASA in freeze stressed wheat seedlings in time- and developmental phase-specific manners. Furthermore, we found that the ABA-independent and ABA-dependent pathways were not completely independent in this study and that ABA-independent stress-responsive genes were also induced by exogenous ABA in at least one stress exposure time point. Comparison of our results with the findings of previous studies showed that plant hormones induce an increase in GSH and ASA levels, possibly by differentially regulating the expression levels of genes encoding ASA–GSH synthesis enzymes. Our results provide the first insights on the molecular mechanism of ABA-induced spring freeze tolerance in wheat.
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影响因子：
4.4
作者：
Song G;Zhang R;Zhang S;Li Y;Gao J;Han X;Chen M;Wang J;Li W;Li G
通讯作者：
Li G
影响因子：
6.4
作者：
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影响因子：
5.6
作者：
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通讯作者：
T. Rakić;Maja Lazarević;Ž. Jovanović;S. Radović;S. Siljak-Yakovlev;B. Stevanović;V. Stevanović
DOI：
10.3835/plantgenome2008.06.0344
发表时间：
2008-07
期刊：
The Plant Genome
影响因子：
--
作者：
B. Koo;B. Bushman;I. Mott
通讯作者：
B. Koo;B. Bushman;I. Mott
影响因子：
5.6
作者：
Wei L;Wang L;Yang Y;Wang P;Guo T;Kang G
通讯作者：
Kang G