Recruitment and diversification of an ecdysozoan family of neuropeptide hormones for black widow spider venom expression.
Recruitment and diversification of an ecdysozoan family of neuropeptide hormones for black widow spider venom expression.
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用于黑寡妇蜘蛛毒液表达的神经肽激素蜕皮动物家族的招募和多样化。
DOI：
10.1016/j.gene.2013.11.054
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发表时间：
2014-02-25
期刊：
影响因子：
3.5
通讯作者：
Garb JE
中科院分区：
文献类型：
--
作者：
McCowan C;Garb JE
由于其强大的生理效应和动态进化，包括毒液表达的同源基因的会聚募集，毒液引起了人们的极大关注。在这里，我们提供了新的证据招募的基因从甲壳动物高血糖激素（CHH）和节肢动物离子转运肽（ITP）的超家族的毒液表达黑寡妇蜘蛛。我们从西方黑寡妇蜘蛛（Latrodectus hesperus）、褐寡妇蜘蛛（L. geometricus）和碗橱蜘蛛（Steatoda grossa）。这些序列与来自其他蜘蛛、蝎子和黄蜂毒液cDNA的同源物以及CHH/ITP神经肽的系统发育分析显示latrodectins是CHH/ITP超家族的衍生成员。这些分析表明，CHH/ITP同系物更广泛的蜘蛛毒液，并招募毒液表达在两个额外的节肢动物谱系。我们还发现，latrodectin 2基因和几乎所有的CHH/ITP基因包括一个2期内含子在相同的位置，支持latrodectin的放置在CHH/ITP超家族。latrodectins的进化分析表明，积极的选择沿着一些序列谱系的情节，和积极的和纯化的选择特定的密码子，支持其功能的重要性寡妇毒液。我们认为，这种改进的理解latrodectin进化通知功能的假设，它在黑寡妇毒液的作用，以及其潜在的收敛招聘毒液表达节肢动物。
Venoms have attracted enormous attention because of their potent physiological effects and dynamic evolution, including the convergent recruitment of homologous genes for venom expression. Here we provide novel evidence for the recruitment of genes from the Crustacean Hyperglycemic Hormone (CHH) and arthropod Ion Transport Peptide (ITP) superfamily for venom expression in black widow spiders. We characterized latrodectin peptides from venom gland cDNAs from the Western black widow spider (Latrodectus hesperus), the brown widow (L. geometricus) and cupboard spider (Steatoda grossa). Phylogenetic analyses of these sequences with homologs from other spider, scorpion and wasp venom cDNAs, as well as CHH/ITP neuropeptides, show latrodectins as derived members of the CHH/ITP superfamily. These analyses suggest that CHH/ITP homologs are more widespread in spider venoms, and were recruited for venom expression in two additional arthropod lineages. We also found that the latrodectin 2 gene and nearly all CHH/ITP genes include a phase 2 intron in the same position, supporting latrodectin’s placement within the CHH/ITP superfamily. Evolutionary analyses of latrodectins suggest episodes of positive selection along some sequence lineages, and positive and purifying selection on specific codons, supporting its functional importance in widow venom. We consider how this improved understanding of latrodectin evolution informs functional hypotheses regarding its role in black widow venom as well as its potential convergent recruitment for venom expression across arthropods.
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影响因子：
3.5
作者：
KIYATKIN, NI;DULUBOVA, IE;GRISHIN, EV
通讯作者：
GRISHIN, EV
影响因子：
4.8
作者：
Dulubova, IE;Krasnoperov, VG;Usherwood, PNR
通讯作者：
Usherwood, PNR
DOI：
10.1016/s0305-0491(98)10088-3
发表时间：
1998-10-01
影响因子：
2.2
作者：
Holz, GG;Habener, JF
通讯作者：
Habener, JF
影响因子：
3.5
作者：
Kordis, D;Gubenek, F
通讯作者：
Gubenek, F
DOI：
10.1111/j.1432-1033.1995.tb20628.x
发表时间：
1995-06-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KIYATKIN, NI;KULIKOVSKAYA, IM;KING, LA
通讯作者：
KING, LA