Peptide-pulsed dendritic cells induce antigen-specific CTL-mediated protective tumor immunity.
Peptide-pulsed dendritic cells induce antigen-specific CTL-mediated protective tumor immunity.
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DOI：
10.1084/jem.183.1.283
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发表时间：
1996-01-01
影响因子：
15.3
通讯作者：
Falo, LD
中科院分区：
文献类型：
--
作者：
Celluzzi, CM;Mayordomo, JI;Storkus, WJ;Lotze, MT;Falo, LD
细胞毒性T淋巴细胞（CTL）是对肿瘤的免疫应答的关键组分。CTL靶向的肿瘤衍生肽抗原被定义用于几种人类肿瘤，并且是用于诱导特异性抗肿瘤免疫的潜在免疫原。树突状细胞（DC）是一种能够在体内引发CTL应答的强有力的抗原呈递细胞（APC）。在这里，我们表明，主要组织相容性复合物I类呈递肽抗原脉冲到树突状APC诱导保护性免疫致死的挑战与抗原基因转染的肿瘤。免疫是抗原特异性的，需要肿瘤靶标表达抗原基因，并通过体内消耗CD8+ T细胞来消除。此外，已经排斥转染的肿瘤的小鼠被保护免于随后用未转染的亲本肿瘤的攻击。这些结果表明，使用抗原脉冲DC的免疫策略可用于诱导肿瘤特异性免疫应答。
Cytotoxic T lymphocytes (CTLs) are a critical component of the immune response to tumors. Tumor-derived peptide antigens targeted by CTLs are being defined for several human tumors and are potential immunogens for the induction of specific antitumor immunity. Dendritic cells (DC) are potent antigen-presenting cells (APCs) capable of priming CTL responses in vivo. Here we show that major histocompatibility complex class I- presented peptide antigen pulsed onto dendritic APCs induces protective immunity to lethal challenge by a tumor transfected with the antigen gene. The immunity is antigen specific, requiring expression of the antigen gene by the tumor target, and is eliminated by in vivo depletion of CD8+ T cells. Furthermore, mice that have rejected the transfected tumor are protected from subsequent challenge with the untransfected parent tumor. These results suggest that immunization strategies using antigen-pulsed DC may be useful for inducing tumor- specific immune responses.
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影响因子：
1.1
作者：
AZIZI, E;BUCANA, C;KRIPKE, ML
通讯作者：
KRIPKE, ML
影响因子：
64.8
作者：
BENJAMIN, RJ;MADRIGAL, JA;PARHAM, P
通讯作者：
PARHAM, P
影响因子：
5.4
作者：
FLAMAND, V;SORNASSE, T;MOSER, M
通讯作者：
MOSER, M
影响因子：
82.9
作者：
MAYORDOMO, JI;ZORINA, T;LOTZE, MT
通讯作者：
LOTZE, MT
影响因子：
6.2
作者：
AUCHINCLOSS, H;GHOBRIAL, RRM;WINN, HJ
通讯作者：
WINN, HJ