Comparison of the protective effects of ferulic acid and its drug-containing plasma on primary cultured neonatal rat cardiomyocytes with hypoxia/reoxygenation injury.
Comparison of the protective effects of ferulic acid and its drug-containing plasma on primary cultured neonatal rat cardiomyocytes with hypoxia/reoxygenation injury.
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DOI：
10.4103/0973-1296.113264
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发表时间：
2013-07
影响因子：
0.7
通讯作者：
Kang TG
中科院分区：
文献类型：
--
作者：
Ren C;Bao YR;Meng XS;Diao YP;Kang TG
为模拟体内缺血再灌注损伤，建立体外培养乳鼠心肌细胞缺氧/复氧损伤模型，采用亚硫酸钠（Na 2S 2 O 4）对原代培养乳鼠心肌细胞进行缺氧1h复氧1h处理。MTT比色法测定细胞活力。用试剂盒检测乳酸脱氢酶（LDH）、Na+-K+-ATP酶和Ca ~（2+）-ATP酶活性。采用显微镜直接观察和细胞内动作电位记录方法，研究阿魏酸及其含药血浆对心肌细胞自律性、电导率、动作电位时程等电生理现象的影响。实验结果表明，二者均能减轻连二亚硫酸钠对心肌细胞的损伤，提高心肌存活率，减少LDH漏出量，提高Na+-K+-ATP酶、Ca ~（2+）-ATP酶活性，增加阿帕（动作电位振幅）、Vmax（最大除极率）和MPD（最大舒张电位）。综上所述，阿魏酸含药血浆对心肌细胞损伤的保护作用优于阿魏酸。
To simulate the ischemia-reperfusion injury in vivo, hypoxia/reoxygenation injury model was established in vitro and primary cultured neonatal rat cardiomyocytes were underwent hypoxia with hydrosulfite (Na2S2O4) for 1 h followed by 1 h reoxygenation. Determination the cell viability by MTT colorimetric assay. We use kit to detect the activity of lactate dehydrogenase (LDH), Na+-K+-ATPase and Ca2+-ATPase. Do research on the effect which ferulic acid and its drug-containing plasma have to self-discipline, conductivity, action potential duration and other electrophysiological phenomena of myocardial cells by direct observation using a microscope and recording method of intracellular action potential. The experimental datum showed that both can reduce the damage hydrosulfite to myocardial cell damage and improve myocardial viability, reduce the amount of LDH leak, increase activity of Na+-K+-ATPase, Ca2+-ATPase, and increase APA (Action potential amplitude), Vmax (Maximum rate of depolarization) and MPD (Maximum potential diastolic). Taken together, therefore, we can get the conclusion that ferulic acid drug-containing plasma has better protective effect injured myocardial cell than ferulic acid.
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