A Simple Method to Isolate and Expand Human Umbilical Cord Derived Mesenchymal Stem Cells: Using Explant Method and Umbilical Cord Blood Serum.
A Simple Method to Isolate and Expand Human Umbilical Cord Derived Mesenchymal Stem Cells: Using Explant Method and Umbilical Cord Blood Serum.
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DOI：
10.15283/ijsc17028
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发表时间：
2017-11-30
影响因子：
2.3
通讯作者：
Aljamali M
中科院分区：
文献类型：
--
作者：
Hassan G;Kasem I;Soukkarieh C;Aljamali M
间充质干细胞（MSC）是多能干细胞，可以从脐带中分离出来，并且由于它们能够分化成各种类型的细胞以及它们的免疫抑制和抗炎特性而在治疗上使用。胎牛血清（FBS），被认为是标准的添加剂时，分离和培养间充质干细胞，有一个主要的限制，其动物来源。在这里，我们采用了一种简单而经济有效的方案，从人脐带组织中分离MSC，而不使用消化酶和用脐带血血清（CBS）代替FBS。通过在CBS或FBS补充的培养基中培养脐带片来分离MSC。采用流式细胞仪、RT-PCR和免疫荧光技术对体外培养的细胞进行形态学观察和多向分化潜能测定。MSC保持形态学、免疫表型、多分化潜能和自我更新能力，与FBS补充培养基相比，在CBS中培养的细胞具有更好的增殖率。我们在这里提出了一种简单，可靠和有效的方法来分离骨髓间充质干细胞从脐带组织，其中细胞保持增殖，分化潜力和免疫表型特性，并可以有效地扩大临床应用。
Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated from umbilical cords and are therapeutically used because of their ability to differentiate into various types of cells, in addition to their immunosuppressive and anti-inflammatory properties. Fetal bovine serum (FBS), considered as the standard additive when isolating and culturing MSCs, has a major limitation related to its animal origin. Here, we employed a simple and economically efficient protocol to isolate MSCs from human umbilical cord tissues without using digestive enzymes and replacing FBS with umbilical cord blood serum (CBS). MSCs were isolated by culturing umbilical cord pieces in CBS or FBS supplemented media. Expansion and proliferation kinetics of cells isolated by explant method in the presence of either FBS or CBS were measured, with morphology and multi-differentiation potential of expanded cells characterized by flow cytometry, RT-PCR, and immunofluorescence. MSCs maintained morphology, immunophenotyping, multi-differentiation potential, and self-renewal ability, with better proliferation rates for cells cultured in CBS compared to FBS supplement media. We here present a simple, reliable and efficient method to isolate MSCs from umbilical cord tissues, where cells maintained proliferation, differentiation potential and immunophenotyping properties and could be efficiently expanded for clinical applications.
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影响因子：
3.3
作者：
Ding, Dah-Ching;Chang, Yu-Hsun;Lin, Shinn-Zong
通讯作者：
Lin, Shinn-Zong
羊水间充质干细胞的分离、培养和鉴定
影响因子：
2.6
作者：
Fei, Xuetao;Jiang, Shan;Ruiz, George
通讯作者：
Ruiz, George
影响因子：
--
作者：
Shetty P;Cooper K;Viswanathan C
通讯作者：
Viswanathan C
影响因子：
4.5
作者：
Hemeda, Hatim;Giebel, Bernd;Wagner, Wolfgang
通讯作者：
Wagner, Wolfgang
DOI：
10.1007/10_2012_134
发表时间：
2013-01-01
期刊：
MESENCHYMAL STEM CELLS: BASICS AND CLINICAL APPLICATION I
影响因子：
--
作者：
Kinzebach, Sven;Bieback, Karen
通讯作者：
Bieback, Karen