Assessment of the genetic relationship between Dictyocaulus species from Bos taurus and Cervus elaphus using complete mitochondrial genomic datasets.
Assessment of the genetic relationship between Dictyocaulus species from Bos taurus and Cervus elaphus using complete mitochondrial genomic datasets.
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DOI：
10.1186/1756-3305-5-241
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发表时间：
2012-10-30
影响因子：
3.2
通讯作者：
Jex AR
中科院分区：
文献类型：
--
作者：
Gasser RB;Jabbar A;Mohandas N;Höglund J;Hall RS;Littlewood DT;Jex AR
网尾线虫属是反刍动物（如牛和鹿）中具有重要兽医意义的圆线虫，可引起严重的支气管炎或肺炎（网尾线虫病或“壳”）。关于某些网尾线虫属物种的有效性及其宿主特异性一直存在争议。在这里，我们测序和特征的线粒体（mt）基因组的网尾线虫胎生（从牛）与网尾线虫sp. cf。来自红鹿（马鹿（Cervus elaphus））的Ecerti，使用MT数据集来评估这些寄生虫和相关寄生虫之间的遗传关系，并预测用于未来群体遗传或分子流行病学研究的标记。mt基因组是从杜父鱼单雄成虫中扩增的。viviparus和Dictyocaulus sp. cf.采用长链PCR技术对来自红鹿的eckerti进行了克隆、测序和生物信息学注释。从两个mt基因组中的每个基因推断的氨基酸序列进行了比较，连接并使用贝叶斯推断（BI）进行系统发育分析，还采用其他圆线虫的数据进行比较。环状mt基因组长度为13，310 bp（D. viviparus）和13，296 bp（网尾藻属（Dictyocaulus sp. cf.）eckerti）大小，每一个都含有12个蛋白编码基因、22个转运RNA基因和2个核糖体RNA基因，与迄今为止测序的其他圆线虫一致。滑动窗口分析确定了mt基因组之间具有高或低水平核苷酸多样性的基因。在预测的mt蛋白质组水平上，D. viviparus和Dictyocaulus sp. cf. eckerti，并且每个物种内的氨基酸序列变异通常远低于物种之间的差异。对所有12个mt蛋白的氨基酸序列数据进行系统发育分析表明，D。viviparus和Dictyocaulus sp. cf.埃克蒂是密切相关的，并分组，以排除超科Metastrongyloidea，Trichostrongyloidea，Ancylostomatoidea和Strongyloidea的选定成员。与以前的核核糖体DNA序列数据的研究结果一致，目前的分析表明，网尾藻sp. cf. eckerti（红鹿）和D.胎生是不同的物种。两个线粒体基因组和蛋白质组中的条形码应该作为未来研究这些和相关网尾虫物种的群体遗传学和/或流行病学的标记。
Dictyocaulus species are strongylid nematodes of major veterinary significance in ruminants, such as cattle and cervids, and cause serious bronchitis or pneumonia (dictyocaulosis or “husk”). There has been ongoing controversy surrounding the validity of some Dictyocaulus species and their host specificity. Here, we sequenced and characterized the mitochondrial (mt) genomes of Dictyocaulus viviparus (from Bos taurus) with Dictyocaulus sp. cf. eckerti from red deer (Cervus elaphus), used mt datasets to assess the genetic relationship between these and related parasites, and predicted markers for future population genetic or molecular epidemiological studies. The mt genomes were amplified from single adult males of D. viviparus and Dictyocaulus sp. cf. eckerti (from red deer) by long-PCR, sequenced using 454-technology and annotated using bioinformatic tools. Amino acid sequences inferred from individual genes of each of the two mt genomes were compared, concatenated and subjected to phylogenetic analysis using Bayesian inference (BI), also employing data for other strongylids for comparative purposes. The circular mt genomes were 13,310 bp (D. viviparus) and 13,296 bp (Dictyocaulus sp. cf. eckerti) in size, and each contained 12 protein-encoding, 22 transfer RNA and 2 ribosomal RNA genes, consistent with other strongylid nematodes sequenced to date. Sliding window analysis identified genes with high or low levels of nucleotide diversity between the mt genomes. At the predicted mt proteomic level, there was an overall sequence difference of 34.5% between D. viviparus and Dictyocaulus sp. cf. eckerti, and amino acid sequence variation within each species was usually much lower than differences between species. Phylogenetic analysis of the concatenated amino acid sequence data for all 12 mt proteins showed that both D. viviparus and Dictyocaulus sp. cf. eckerti were closely related, and grouped to the exclusion of selected members of the superfamilies Metastrongyloidea, Trichostrongyloidea, Ancylostomatoidea and Strongyloidea. Consistent with previous findings for nuclear ribosomal DNA sequence data, the present analyses indicate that Dictyocaulus sp. cf. eckerti (red deer) and D. viviparus are separate species. Barcodes in the two mt genomes and proteomes should serve as markers for future studies of the population genetics and/or epidemiology of these and related species of Dictyocaulus.
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DOI：
10.1073/pnas.96.8.4482
发表时间：
1999-04-13
影响因子：
11.1
作者：
Duret, L;Mouchiroud, D
通讯作者：
Mouchiroud, D
影响因子：
3.3
作者：
BLOUIN, MS;DAME, JB;COURTNEY, CH
通讯作者：
COURTNEY, CH
影响因子：
3.2
作者：
Dolores Bargues, M.;Artigas, Patricio;Mas-Coma, Santiago
通讯作者：
Mas-Coma, Santiago
影响因子：
4
作者：
Blouin, MS
通讯作者：
Blouin, MS
影响因子：
10.7
作者：
Blouin, MS;Yowell, CA;Dame, JB
通讯作者：
Dame, JB