Analysis of biodegradation performance of furfural and 5-hydroxymethylfurfural by Amorphotheca resinae ZN1.
Analysis of biodegradation performance of furfural and 5-hydroxymethylfurfural by Amorphotheca resinae ZN1.
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紫穗槐ZN1对糠醛和5-羟甲基糠醛的生物降解性能分析
DOI：
10.1186/1754-6834-7-51
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发表时间：
2014-04-05
影响因子：
6.3
通讯作者：
Bao J
中科院分区：
文献类型：
--
作者：
Ran H;Zhang J;Gao Q;Lin Z;Bao J
糠醛和5-羟甲基糠醛（HMF）是木质纤维素在预处理过程中的降解产物，对后续的酶水解和发酵过程有显著的抑制作用。生物解毒真菌Amorphotheca resinae ZN 1具有良好的降解木质纤维素衍生抑制剂的能力，有助于发酵过程获得高产的乙醇和生物化学品。分析了A. resinae ZN 1菌株对糠醛和羟甲基糠醛的降解性能研究表明，ZN 1菌株对糠醛和羟甲基糠醛的降解能力较好，且能快速、彻底地去除糠醛和羟甲基糠醛，有利于后续的乙醇发酵。resinae ZN 1在不同培养条件下的中间代谢产物的捕获研究。A. resinae ZN 1在好氧条件下可将糠醛/HMF同时转化为糠醇/HMF和糠酸/HMF，而在厌氧条件下只生成相应的糠醇/HMF。葡萄糖的存在促进了A. resinae ZN 1和细胞团生长速率。值得注意的是，在糠醛或HMF降解至低阈值浓度之前葡萄糖未被消耗。这一结果表明，糠醛或HMF是A. ZN 1比葡萄糖更高。这一特性有助于糠醛和HMF的脱毒过程在不消耗葡萄糖的情况下进行。对ZN 1树脂进行了研究。供氧对A.脂树ZN 1。糠醛或HMF比葡萄糖具有更好的底物利用能力。脂树ZN 1。本研究为脱毒强化和菌种改良提供了重要信息。
BackgroundFurfural and 5-hydroxymethylfurfural (HMF) are the degradation products of lignocellulose during pretreatment operations and significantly inhibit the consequent enzymatic hydrolysis and fermentation processes. The biodetoxification fungus Amorphotheca resinae ZN1 had demonstrated its excellent capacity on degrading lignocellulose derived inhibitors and helped the fermentation processes to achieve high yield of ethanol and biochemicals. Analysis of the biological degradation performance of furfural and HMF by A. resinae ZN1 will provide essential information for their fast and complete removal from the pretreated lignocellulose materials and facilitate the consequent ethanol fermentation.ResultsThe degradation performance of furfural and HMF by A. resinae ZN1 was investigated by capturing intermediate metabolic products at various culture conditions. A. resinae ZN1 converts furfural/HMF into furfuryl/HMF alcohols and furoic/HMF acids simultaneously at aerobic condition, and only the corresponding furfuryl/HMF alcohols are obtained at anaerobic condition. The existence of glucose accelerates the degradation rate of furfural and HMF by A. resinae ZN1 and the cell mass growth rate aerobically. Remarkably, glucose is not consumed before furfural or HMF is degraded to a low threshold concentration. The finding suggests that furfural or HMF has a substrate priority of utilization by A. resinae ZN1 than glucose. This property may help the detoxification of furfural and HMF to be operated without consuming glucose.ConclusionsThe biological degradation performance of furfural and HMF by A. resinae ZN1 was investigated experimentally. Oxygen supply is important on the complete biodegradation of furfural and HMF by A. resinae ZN1. Furfural or HMF has the priority of substrate utilization than glucose by A. resinae ZN1. This study provided important information for detoxification enhancement and strain modification.
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影响因子：
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作者：
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