Regulation of innate immune function in bovine oviduct epithelial cells in culture: the homeostatic role of epithelial cells in balancing Th1/Th2 response.
Regulation of innate immune function in bovine oviduct epithelial cells in culture: the homeostatic role of epithelial cells in balancing Th1/Th2 response.
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DOI：
10.1262/jrd.2013-036
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Miyamoto A
中科院分区：
文献类型：
--
作者：
Kowsar R;Hambruch N;Liu J;Shimizu T;Pfarrer C;Miyamoto A
本研究旨在探讨牛输卵管上皮细胞(BOEC)培养中上皮细胞在调节天然免疫中的作用。本文研究了在排卵前期观察到的不同浓度的大肠杆菌脂多糖(LPS)及其与卵巢类固醇、雌二醇(E_2)、孕酮(P_4)、黄体生成素(L H)的相互作用对BOEC免疫应答的影响。输卵管组织免疫组织化学显示Toll样受体-4(TLR-4)和TLR-2在输卵管上皮细胞中有较强的表达。10 ng/mlLPS刺激TLR4、环氧合酶2(COX-2)、核因子kappaB抑制物A(NFKBIA)、白介素1β(IL-1β)和肿瘤坏死因子α(TNF一α)的表达，提示早期的促炎反应。100 ng/mlLPS不能诱导这些基因的表达，但可刺激TLR-2、IL-10、IL-4和微粒体前列腺素E合成酶-1(mPGES-1)的表达和PGE2的分泌，表明有抗炎反应。卵巢激素和黄体生成素完全阻断脂多糖(10 ng/ml)诱导的TLR4、IL-1β和肿瘤坏死因子-α的表达以及脂多糖(100 ng/mlLPS)诱导的TLR2的表达。综上所述，这项研究表明在BOEC中存在一个早期信号系统来对感染做出反应。此外，卵巢类固醇和黄体生成素可能在诱导动态平衡和控制过度活跃的促炎反应中发挥关键作用，这些反应对上皮细胞、精子和胚胎不利。
This study aimed to investigate the role of epithelial cells in regulating innate immunity in bovine oviduct epithelial cell (BOEC) culture. We studied the effect of Escherichia coli lipopolysaccharide (LPS) and its interaction with ovarian steroids, estradiol (E2) and progesterone (P4), and luteinizing hormone (LH) at concentrations observed during the preovulatory period on immune responses in BOEC culture. Immunohistochemistry of oviduct tissue showed intensive expression of Toll-like receptor-4 (TLR-4) and TLR-2 in epithelial cells. A dose of 10 ng/ml LPS stimulated TLR-4, cyclooxygenase-2 (COX-2), nuclear factor kappa B inhibitor A (NFKBIA), interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) expression, indicating an early pro-inflammatory response. A dose of 100 ng/ml LPS did not induce expression of these genes but stimulated TLR-2, IL-10,IL-4 and microsomal prostaglandin E synthase-1 (mPGES-1) expression and PGE2 secretion, indicating an anti-inflammatory response. Ovarian steroids and LH completely block LPS (10 ng/ml)-induced TLR-4, IL-1β and TNF-α expression as well as LPS (100 ng/ml)-induced TLR-2 expression. Taken together, this study suggests the existence of an early signaling system to respond to infection in the BOEC. In addition, ovarian steroids and LH may play a critical role in inducing homeostasis and in controlling hyperactive pro-inflammatory responses detrimental to epithelial cells, sperm and the embryo.
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DOI：
10.1530/rep-07-0229
发表时间：
2007-11
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Herath S;Williams EJ;Lilly ST;Gilbert RO;Dobson H;Bryant CE;Sheldon IM
通讯作者：
Sheldon IM
DOI：
10.1016/s0002-9378(96)70035-2
发表时间：
1996-11-01
影响因子：
9.8
作者：
Ault, KA;Tawfik, OW;Terranova, PT
通讯作者：
Terranova, PT
影响因子：
2.8
作者：
Koelle, S.;Reese, S.;Kummer, W.
通讯作者：
Kummer, W.
影响因子：
4.4
作者：
Faure, E;Thomas, L;Arditi, M
通讯作者：
Arditi, M
影响因子：
56.9
作者：
Aliprantis, AO;Yang, RB;Zychlinsky, A
通讯作者：
Zychlinsky, A