Deep analysis of immune response and metabolic signature in children with food protein induced enterocolitis to cow's milk.
Deep analysis of immune response and metabolic signature in children with food protein induced enterocolitis to cow's milk.
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DOI：
10.1186/s13601-018-0224-9
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发表时间：
2018
影响因子：
4.4
通讯作者：
Dupont C
中科院分区：
文献类型：
--
作者：
Adel-Patient K;Lezmi G;Castelli FA;Blanc S;Bernard H;Soulaines P;Dumond P;Ah-Leung S;Lageix F;de Boissieu D;Cortes-Perez N;Hazebrouck S;Fenaille F;Junot C;Dupont C
食物蛋白诱发的小肠结肠炎综合征 (FPIES) 被认为是一种非 IgE 介导的食物过敏。然而，其发病机制仍知之甚少，且缺乏生物标志物。我们的目的是对牛奶 (CM)-FPIES 儿童的体液和细胞免疫反应进行深入表征，并研究是否存在 FPIES 代谢组学特征。患有 CM-FPIES 的儿童和患有 IgE 介导的 CM 过敏 (IgE-CMA) 的对照受试者（均避免 CM）在口服食物挑战当天被招募。在攻击之前收集血样。使用耐 CM 花生过敏患者的血浆（IgE-PA，不避免 CM）作为额外对照，测量血浆中针对各种乳清和酪蛋白过敏原及其胃十二指肠消化产物的 IgE、IgG1-4、IgA、IgM 和 IgD 的总水平和特异性水平。用不同的 CM 过敏原刺激 PBMC 后，分析细胞因子分泌和细胞增殖。使用液相色谱与高分辨率质谱联用获得血浆样品的代谢组学特征。包括 9 名患有 CM-FPIES 的儿童和 12 名对照受试者（6 名 IgE-CMA 和 6 名 IgE-PA）。在患有 CM-FPIES 的儿童中，总 Ig 浓度低于对照受试者，针对 CM 成分的特异性 Ig 很弱甚至无法检测到，并且没有检测到针对 CM 消化产物的特异性 IgE。此外，在 CM-FPIES 患者中，我们在过敏原重新激活后没有发现任何 Th 细胞增殖或相关细胞因子分泌，而在 IgE-CMA 儿童中明显发现了这种反应。 CM 过敏患者之间的血浆代谢特征不同，与 IgE-CMA 患者相比，CM-FPIES 中各种脂肪酸的浓度显着较低，而初级代谢物（例如氨基酸）的浓度较高。在 CM-FPIES 中，体液和细胞特异性免疫反应都很弱或不存在，这与 CM 回避无关。在 CM-FPIES 患者中鉴定出代谢组学特征，可能有助于该疾病的诊断和治疗。本文的在线版本 (10.1186/s13601-018-0224-9) 包含补充材料，可供授权用户使用。
Food Protein-Induced Enterocolitis Syndrome (FPIES) is considered to be a non-IgE mediated food allergy. However, its pathogenesis remains poorly understood and biomarkers are lacking. We aimed to perform in-depth characterization of humoral and cellular immune responses in children with cow’s milk (CM)-FPIES and investigated whether there is a FPIES metabolomic signature. Children with CM-FPIES and control subjects with an IgE-mediated CM allergy (IgE-CMA), both avoiding CM, were recruited on the day of an oral food challenge. Blood samples were collected before the challenge. Total and specific levels of IgE, IgG1-4, IgA, IgM and IgD to various whey and casein allergens and to their gastroduodenal digestion products were measured in plasma, using plasma from CM-tolerant peanut allergic patients (IgE-PA, not avoiding CM) as additional controls. Cytokine secretion and cellular proliferation were analyzed after stimulation of PBMC with different CM allergens. Metabolomic profiles were obtained for plasma samples using liquid chromatography coupled to high-resolution mass spectrometry. Nine children with CM-FPIES and 12 control subjects (6 IgE-CMA and 6 IgE-PA) were included. In children with CM-FPIES, total Ig concentrations were lower than in control subjects, specific Ig against CM components were weak to undetectable, and no specific IgE against CM digestion products were detected. Moreover, in CM-FPIES patients, we did not find any Th cell proliferation or associated cytokine secretion after allergen reactivation, whereas such responses were clearly found in children with IgE-CMA. Plasma metabolic profiles were different between CM allergic patients, with significantly lower concentrations of various fatty acids and higher concentrations of primary metabolites such as amino acids in CM-FPIES compared to IgE-CMA patients. In CM-FPIES, both humoral and cellular specific immune responses are weak or absent, and this is not related to CM avoidance. A metabolomic signature was identified in patients with CM-FPIES that may be useful for the diagnosis and management of this disease. The online version of this article (10.1186/s13601-018-0224-9) contains supplementary material, which is available to authorized users.
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影响因子：
14.2
作者：
Berin, M. Cecilia
通讯作者：
Berin, M. Cecilia
影响因子：
12.4
作者：
Bernard, H;Paty, E;Scheinmann, P
通讯作者：
Scheinmann, P
DOI：
10.1016/j.jchromb.2014.04.025
发表时间：
2014-09-01
影响因子：
3
作者：
Boudah, Samia;Olivier, Marie-Francoise;Junot, Christophe
通讯作者：
Junot, Christophe
影响因子：
4.3
作者：
Bernard, Herve;Drumare, Marie-Francoise;Wal, Jean-Michel
通讯作者：
Wal, Jean-Michel
影响因子：
14.2
作者：
GRASSI, J;DIDIERLAURENT, A;STADLER, BM
通讯作者：
STADLER, BM