Electric field stimulates production of highly conductive microbial OmcZ nanowires.
Electric field stimulates production of highly conductive microbial OmcZ nanowires.
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DOI：
10.1038/s41589-020-0623-9
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发表时间：
2020-10
影响因子：
14.8
通讯作者：
Malvankar NS
中科院分区：
文献类型：
--
作者：
Yalcin SE;O'Brien JP;Gu Y;Reiss K;Yi SM;Jain R;Srikanth V;Dahl PJ;Huynh W;Vu D;Acharya A;Chaudhuri S;Varga T;Batista VS;Malvankar NS
多功能生命材料因其强大的自我修复和复制能力而备受关注。然而，大多数天然材料缺乏电子功能。在这里，我们证明，应用于发电的硫还原地杆菌生物膜的电场可以刺激以前未知的细胞色素 OmcZ 纳米线的产生，其电导率 (30 S/cm) 比自然环境中重要的细胞色素 OmcS 纳米线高 1,000 倍，刚度 (1.5 GPa) 高 3 倍。使用基于化学成像的多模态纳米光谱，我们将蛋白质结构与功能关联起来，并观察到 pH 诱导的单个纳米线构象转变为 β-折叠，由于血红素基团的 π 堆积增强，其刚度和电导率增加了 100 倍；计算模型和批量光谱研究进一步证实了这一点。这些纳米线可以将机械和化学刺激转换为电信号，以执行传感、合成和能量生产。这些生物产生的高导电蛋白质纳米线的发现可能有助于指导生物和电子系统之间无缝、双向接口的开发。
Multifunctional living materials are attractive due to their powerful ability to self-repair and replicate. However, most natural materials lack electronic functionality. Here we show that an electric field, applied to electricity-producing Geobacter sulfurreducens biofilms, stimulates production of previously unknown cytochrome OmcZ nanowires with 1,000-fold higher conductivity (30 S/cm), and 3-fold higher stiffness (1.5 GPa), than the cytochrome OmcS nanowires that are important in natural environments. Using chemical imaging-based multimodal nanospectroscopy, we correlate protein structure with function, and observe pH-induced conformational switching to β-sheets in individual nanowires, which increases their stiffness and conductivity by 100-fold due to enhanced π-stacking of heme groups; this was further confirmed by computational modelling and bulk spectroscopic studies. These nanowires can transduce mechanical and chemical stimuli into electrical signals to perform sensing, synthesis and energy production. These findings of biologically-produced, highly-conductive protein nanowires may help to guide the development of seamless, bidirectional interfaces between biological and electronic systems.
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影响因子：
64.8
作者：
Lee, K;Cho, S;Lee, SH
通讯作者：
Lee, SH
影响因子：
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通讯作者：
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