FRA-1 protein overexpression is a feature of hyperplastic and neoplastic breast disorders.
FRA-1 protein overexpression is a feature of hyperplastic and neoplastic breast disorders.
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DOI：
10.1186/1471-2407-7-17
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发表时间：
2007-01-25
期刊：
影响因子：
3.8
通讯作者：
Fusco, Alfredo
中科院分区：
文献类型：
--
作者：
Chiappetta, Gennaro;Ferraro, Angelo;Botti, Gerardo;Monaco, Mario;Pasquinelli, Rosa;Vuttariello, Emilia;Arnaldi, Liliane;Di Bonito, Maurizio;D'Aiuto, Giuseppe;Pierantoni, Giovanna Maria;Fusco, Alfredo
Fos相关抗原1（Fos-related antigen 1，FRA-1）是一种即早基因，编码AP-1家族转录因子，参与细胞增殖、分化、凋亡等生物学过程。fra-1基因的过度表达在细胞转化过程中起重要作用，我们的前期研究表明FRA-1蛋白检测可作为甲状腺肿瘤诊断的有用工具。本研究通过免疫组化、Western blot、RT-PCR和qPCR检测FRA-1蛋白在乳腺良恶性肿瘤组织中的表达，探讨其在乳腺肿瘤疾病诊断和预后判断中的价值。采用免疫组化、Western blot、RT-PCR和qPCR方法检测70例乳腺癌和30例乳腺良性疾病中FRA-1蛋白的表达。FRA-1蛋白在所有癌组织中均有表达，在细胞核中呈强阳性。在纤维腺瘤中也发现了阳性染色，但在这种情况下，染色存在于细胞核和细胞质中，阳性细胞数低于癌。从乳腺增生的分析中获得了类似的结果，典型和非典型乳腺病变之间的FRA-1表达水平没有差异;然而，FRA-1蛋白定位在非典型增生中主要是核。原位乳腺癌显示FRA-1蛋白表达的模式非常相似，观察到的非典型增生。相反，在用作对照的6个正常乳腺组织样品中未检测到FRA-1蛋白。RT-PCR和qPCR分析证实了这些结果。分析细针穿刺活检（FNAB）样品中的FRA-1表达获得了类似的结果。这里显示的数据表明，FRA-1的表达，包括其细胞内定位，可能被认为是一个有用的标记增生性和肿瘤增生性乳腺疾病。
Fos-related antigen 1 (FRA-1) is an immediate early gene encoding a member of AP-1 family of transcription factors involved in cell proliferation, differentiation, apoptosis, and other biological processes. fra-1 gene overexpression has an important role in the process of cellular transformation, and our previous studies suggest FRA-1 protein detection as a useful tool for the diagnosis of thyroid neoplasias. Here we investigate the expression of the FRA-1 protein in benign and malignant breast tissues by immunohistochemistry, Western blot, RT-PCR and qPCR analysis, to evaluate its possible help in the diagnosis and prognosis of breast neoplastic diseases. We investigate the expression of the FRA-1 protein in 70 breast carcinomas and 30 benign breast diseases by immunohistochemistry, Western blot, RT-PCR and qPCR analysis. FRA-1 protein was present in all of the carcinoma samples with an intense staining in the nucleus. Positive staining was also found in most of fibroadenomas, but in this case the staining was present both in the nucleus and cytoplasm, and the number of positive cells was lower than in carcinomas. Similar results were obtained from the analysis of breast hyperplasias, with no differences in FRA-1 expression level between typical and atypical breast lesions; however the FRA-1 protein localization is mainly nuclear in the atypical hyperplasias. In situ breast carcinomas showed a pattern of FRA-1 protein expression very similar to that observed in atypical hyperplasias. Conversely, no FRA-1 protein was detectable in 6 normal breast tissue samples used as controls. RT-PCR and qPCR analysis confirmed these results. Similar results were obtained analysing FRA-1 expression in fine needle aspiration biopsy (FNAB) samples. The data shown here suggest that FRA-1 expression, including its intracellular localization, may be considered a useful marker for hyperplastic and neoplastic proliferative breast disorders.
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影响因子：
8
作者：
Belguise, K;Kersual, N;Chalbos, D
通讯作者：
Chalbos, D
影响因子：
11.5
作者：
Chiappetta, G;Botti, G;Fusco, A
通讯作者：
Fusco, A
影响因子：
2.1
作者：
Koshkin, AA;Singh, SK;Wengel, J
通讯作者：
Wengel, J
影响因子：
11.4
作者：
Vallone, D;Battista, S;Verde, P
通讯作者：
Verde, P
DOI：
10.1093/jnci/85.11.892
发表时间：
1993-06-02
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
FEUER, EJ;WUN, LM;TONG, T
通讯作者：
TONG, T